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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1996-10-21
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pubmed:databankReference |
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pubmed:abstractText |
We constructed a hybrid Moloney murine leukaemia virus (MoMLV) bearing both a chimeric CD4 and the wild-type MoMLV envelope protein (Env) on its surface. The chimeric molecule, consisting of a surface domain of CD4 and the C-terminal two-thirds of MLV Env, was expressed on the cell surface. When expressed in MoMLV-infected cells, the CD4-Env chimera was incorporated into the virion as efficiently as the wild-type MoMLV Env. The hybrid MoMLV could infect human HeLa cells (although not with high efficiency) only if the cells were expressing human immunodeficiency virus type 1 genome. This method of ligand incorporation into a virion may lead to a development of a cell-specific retroviral vector for targeting gene therapy.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0022-1317
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
76 ( Pt 12)
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3165-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8847525-3T3 Cells,
pubmed-meshheading:8847525-Animals,
pubmed-meshheading:8847525-Antigens, CD4,
pubmed-meshheading:8847525-Cell Membrane,
pubmed-meshheading:8847525-Friend murine leukemia virus,
pubmed-meshheading:8847525-Gene Expression,
pubmed-meshheading:8847525-Gene Products, env,
pubmed-meshheading:8847525-Gene Targeting,
pubmed-meshheading:8847525-Genetic Vectors,
pubmed-meshheading:8847525-HIV-1,
pubmed-meshheading:8847525-HeLa Cells,
pubmed-meshheading:8847525-Humans,
pubmed-meshheading:8847525-Mice,
pubmed-meshheading:8847525-Molecular Sequence Data,
pubmed-meshheading:8847525-Moloney murine leukemia virus,
pubmed-meshheading:8847525-RNA, Messenger,
pubmed-meshheading:8847525-RNA, Viral,
pubmed-meshheading:8847525-Recombinant Fusion Proteins,
pubmed-meshheading:8847525-Virion
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pubmed:year |
1995
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pubmed:articleTitle |
Targeted infection of a retrovirus bearing a CD4-Env chimera into human cells expressing human immunodeficiency virus type 1.
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pubmed:affiliation |
Department of Bacteriology, Faculty of Medicine, University of Tokyo, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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