Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1996-12-31
|
| pubmed:abstractText |
A two-step methylcellulose culture provided a method to study the differentiation of murine lymphohematopoietic progenitors. In the presence of two cytokines, one from a group consisting of Steel factor (SF) and flt3/flk2 ligand (FL) and the other from a group consisting of interleukin 6 (IL-6), G-CSF, IL-11 and IL-12, murine lymphohematopoietic progenitors proliferated and generated not only myeloid lineage cells but also committed B cell progenitors. Although somewhat less effectively than SF and FL, IL-4 also synergized with IL-6 or IL-11 in support of B lymphopoiesis. This early process of B lymphopoiesis appears to proceed through three stages: lymphohematopoietic proliferative stage, commitment stage and early B lymphoid proliferative stage. Surprisingly, IL-3 could neither replace nor act synergistically with SF, IL-4 or FL in maintaining the B lymphoid potential of the cells in the primary culture, although IL-3 was very effective in support of multilineage myeloid colony formation. In addition, when added to permissive cytokine combinations, IL-3 inhibited development of the B cell lineage. After screening available lymphohematopoietic cytokines, it was found that IL-1 (both alpha and beta) also has similar inhibitory effects on early B lymphopoiesis. Studies using in vivo transfer of primary colonies suggested that cytokine regulation of commitment to T cell lineage may also be similar to that of B cell lineage.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
1066-5099
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
14
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
369-75
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading | |
| pubmed:year |
1996
|
| pubmed:articleTitle |
Cytokine regulation of early lymphohematopoietic development.
|
| pubmed:affiliation |
Department of Medicine, Medical University of South Carolina, Charleston, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Review
|