|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
7
|
|
pubmed:dateCreated |
1996-11-7
|
|
pubmed:abstractText |
The mi locus of mice encodes a member of the basic-helix-loop-helix-leucine zipper (bHLH-Zip) protein family of transcription factors (hereafter called MITF). Because the expression of the mouse mast cell protease 6 (MMCP-6) gene is remarkably reduced in mast cells of mi/mi mutant mice, we investigated the effect of MITF on the transcription of the MMCP-6 gene. First, we introduced the normal (+) MITF cDNA into mi/mi cultured mast cells using the retroviral vector. Overexpression of +-MITF but not mi-MITF normalized the expression of the MMCP-6 gene, indicating the involvement of +-MITF in the MMCP-6 gene transactivation. Second, we analyzed the promoter of the MMCP-6 gene by the transient cotransfection assay. The luciferase construct under the control of the MMCP-6 promoter and the cDNA encoding +-MITF or mi-MITF were cotransfected into NIH/ 3T3 fibroblasts. The coexpression of +-MITF but not mi-MITF increased the luciferase activity 10-fold. We found a CACATG and a CATCTG motif in the MMCP-6 promoter, both of which are generally recognized by bHLH-Zip-type transcription factors. We also found a GACCTG motif that was strongly bound by +-MITF. These three motifs were necessary for the 10-fold transactivation ability of the MMCP-6 promoter by +-MITF. Mutations of each motif significantly reduced the transactivation, suggesting that +-MITF directly transactivated the MMCP-6 gene through these three motifs.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
AIM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Mcpt6 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Microphthalmia-Associated...,
http://linkedlifedata.com/resource/pubmed/chemical/Mitf protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Serine Endopeptidases,
http://linkedlifedata.com/resource/pubmed/chemical/Tpsab1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Tryptases
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Oct
|
|
pubmed:issn |
0006-4971
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
1
|
|
pubmed:volume |
88
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
2488-94
|
|
pubmed:dateRevised |
2008-11-21
|
|
pubmed:meshHeading |
pubmed-meshheading:8839840-3T3 Cells,
pubmed-meshheading:8839840-Amino Acid Sequence,
pubmed-meshheading:8839840-Animals,
pubmed-meshheading:8839840-Base Sequence,
pubmed-meshheading:8839840-DNA, Complementary,
pubmed-meshheading:8839840-DNA-Binding Proteins,
pubmed-meshheading:8839840-Enzyme Induction,
pubmed-meshheading:8839840-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:8839840-Genes, Reporter,
pubmed-meshheading:8839840-Mast Cells,
pubmed-meshheading:8839840-Mice,
pubmed-meshheading:8839840-Mice, Mutant Strains,
pubmed-meshheading:8839840-Microphthalmia-Associated Transcription Factor,
pubmed-meshheading:8839840-Molecular Sequence Data,
pubmed-meshheading:8839840-Peptide Fragments,
pubmed-meshheading:8839840-Promoter Regions, Genetic,
pubmed-meshheading:8839840-Recombinant Fusion Proteins,
pubmed-meshheading:8839840-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:8839840-Sequence Alignment,
pubmed-meshheading:8839840-Serine Endopeptidases,
pubmed-meshheading:8839840-Transcription Factors,
pubmed-meshheading:8839840-Transcriptional Activation,
pubmed-meshheading:8839840-Transfection,
pubmed-meshheading:8839840-Tryptases
|
|
pubmed:year |
1996
|
|
pubmed:articleTitle |
Regulation of mouse mast cell protease 6 gene expression by transcription factor encoded by the mi locus.
|
|
pubmed:affiliation |
Department of Pathology, Osaka University Medical School, Japan.
|
|
pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|
