Linked Life Data

8836164

Source:http://linkedlifedata.com/resource/pubmed/id/8836164

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1996-11-13
pubmed:abstractText
In attempting to elucidate the molecular basis of the expression of alpha 2,6-sialyltransferase (alpha 2,6-ST) in jejunal explants of 7-day-old rats during cultivation, the total jejunal RNA was analysed by hybridization using a cDNA clone encoding rat liver alpha 2,6-ST. Under cultivation in both serum-free and serum-containing media jejunal alpha 2,6-ST mRNA closely paralleled the bound (100,000 g pellet) as well as the soluble (100,000 g supernatant) alpha 2,6-ST activity, the correlation coefficients being 0.976 and 0.816, respectively. Dexamethasone (Dx) treatment enhanced alpha 2,6-ST mRNA and membrane-bound alpha 2,6-ST activity in close correlation. Jejunal alpha 2,6-ST mRNA is sensitive to actinomycin D and is lost with apparently identical kinetics in Dx-stimulated and control explants, suggesting that regulation by Dx may be exerted by altering the rate of mRNA synthesis. Dx-dependent activation resulted in elevation of the 4.3-Kb mRNA and can be inhibited by the antiglucocorticoid onapristone, demonstrating the participation of the glucocorticoid receptor pathway.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0960-0760
pubmed:author
pubmed:issnType
Print
pubmed:volume
58
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
289-97
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Coordinate expression of beta-galactoside alpha 2,6-sialyltransferase mRNA and enzyme activity in suckling rat jejunum cultured in different media: transcriptional induction by dexamethasone.
pubmed:affiliation
Institute of Physiology, Czech Academy of Sciences, Prague, Czech Republic.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't