Linked Life Data

8825783

Source:http://linkedlifedata.com/resource/pubmed/id/8825783

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1996-12-5
pubmed:abstractText
Transcription of the Escherichia coli dnaA gene, encoding DnaA protein required for initiation of chromosomal DNA replication at oriC in E. coli, starts from two promoters, 1P and 2P. Gel-shift and DNase I-protection assays revealed that IciA protein, an inhibitor of initiation of in vitro E. coli chromosomal DNA replication at oriC, bound to two sites in the dnaA promoter region. One site is located upstream of promoter 1P, and the second is located downstream of promoter 2P. Whereas IciA protein did not affect transcription from the promoter 2P, transcription from the promoter 1P was specifically enhanced by IciA protein in vivo and in vitro. DnaA protein bound to the DnaA box between the two promoters 1P and 2P, acts as an transcriptional repressor. Under this condition, IciA protein counteracted the repressive effect of DnaA protein on the promoter 1P. These findings suggest that IciA protein may regulate the initiation of chromosomal DNA replication at oriC by controlling expression of the dnaA gene, as well as by inhibiting the initiation of chromosomal DNA replication at oriC.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0950-382X
pubmed:author
pubmed:issnType
Print
pubmed:volume
19
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
389-96
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Transcriptional activation of the dnaA gene encoding the initiator for oriC replication by IciA protein, an inhibitor of in vitro oriC replication in Escherichia coli.
pubmed:affiliation
Institute for Molecular Biology and Genetics, Seoul National University, Korea.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't