Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1996-12-10
|
| pubmed:abstractText |
The aims of this study were to produce and characterize mouse monoclonal antiidiotypic antibodies (AIAs) that specifically bind human antipig (anti-alphaGal) antibodies and to select those AIAs that neutralize the cytotoxicity of human or baboon serum to pig (PK15) cells. Mice were immunized with human anti-pig antibodies, and hybridomas were produced using conventional techniques. From a total of 480 clones, 11 produced AIAs that bound with high affinity to human anti-alpha Gal IgG and F(ab')2 fragments, and individually reduced serum cytotoxicity to pig cells by 40 - 90%. Seven of the AIAs also bound to human peripheral B lymphocytes (that express the same idiotypes as the antibodies produce). Several combinations of two or three AIAs provided 100% protection of PK15 cells. Selected AIAs injected intravenously into baboons reduced the cytotoxicity of subsequently drawn sera by 50--80% for >48 hr. The cytotoxicity studies also indicated that there are at least two dominant idiotypes expressed in the human anti-alphaGal population. We conclude that AIAs against anti-alphaGal antibodies could be successfully used in 1) preoperative characterization of a recipient's anti-alphaGal profile, (2) prevention of hyperacute rejection of a pig organ, and (3) specific immunosuppression through elimination of anti-alphaGal-producing B lymphocytes.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Anti-Idiotypic,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Heterophile,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin Fab Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin G,
http://linkedlifedata.com/resource/pubmed/chemical/Trisaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/alpha-galactosyl epitope
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0041-1337
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
27
|
| pubmed:volume |
62
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
837-43
|
| pubmed:dateRevised |
2005-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:8824486-Animals,
pubmed-meshheading:8824486-Antibodies, Anti-Idiotypic,
pubmed-meshheading:8824486-Antibodies, Heterophile,
pubmed-meshheading:8824486-Antibodies, Monoclonal,
pubmed-meshheading:8824486-Antibody-Dependent Cell Cytotoxicity,
pubmed-meshheading:8824486-Antigen-Antibody Reactions,
pubmed-meshheading:8824486-Cross Reactions,
pubmed-meshheading:8824486-Female,
pubmed-meshheading:8824486-Humans,
pubmed-meshheading:8824486-Immunoglobulin Fab Fragments,
pubmed-meshheading:8824486-Immunoglobulin G,
pubmed-meshheading:8824486-Mice,
pubmed-meshheading:8824486-Mice, Inbred BALB C,
pubmed-meshheading:8824486-Papio,
pubmed-meshheading:8824486-Species Specificity,
pubmed-meshheading:8824486-Swine,
pubmed-meshheading:8824486-Transplantation, Heterologous,
pubmed-meshheading:8824486-Trisaccharides
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
Monoclonal antiidiotypic antibodies neutralize cytotoxic effects of anti-alphaGal antibodies.
|
| pubmed:affiliation |
Oklahoma Medical Research Foundation, Oklahoma City, USA.
|
| pubmed:publicationType |
Journal Article
|