Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1996-11-1
pubmed:abstractText
Hippocampal organotypic slice cultures maintained 10-20 days in vitro express a high level of the polysialylated embryonic form of neural cell adhesion molecule (NCAM) (PSA-NCAM). Treatment of the cultures with endoneuraminidase-N selectively removed polysialic acid (PSA) from NCAM and completely prevented induction of long-term potentiation (LTP) and long-term depression (LTD) without affecting cellular or synaptic parameters. Similarly, slices prepared from transgenic mice lacking the NCAM gene exhibited a decaying LTP. No inhibition of N-methyl-D-aspartic acid receptor-dependent synaptic responses was detected. Washout of the enzyme resulted in reexpression of PSA immunoreactivity which correlated with a complete recovery of LTP and LTD. This reexpression was blocked by TTX and low calcium and enhanced by bicuculline. Taken together, these results indicate that neuronal activity regulates the expression of PSA-NCAM at the synapse and that this expression is required for the induction of synaptic plasticity.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0896-6273
pubmed:author
pubmed:issnType
Print
pubmed:volume
17
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
413-22
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:8816705-Animals, pubmed-meshheading:8816705-Animals, Newborn, pubmed-meshheading:8816705-Electrophysiology, pubmed-meshheading:8816705-Glycoside Hydrolases, pubmed-meshheading:8816705-Hippocampus, pubmed-meshheading:8816705-Immunohistochemistry, pubmed-meshheading:8816705-Long-Term Potentiation, pubmed-meshheading:8816705-Mice, pubmed-meshheading:8816705-Mice, Mutant Strains, pubmed-meshheading:8816705-Microscopy, Immunoelectron, pubmed-meshheading:8816705-N-Acetylneuraminic Acid, pubmed-meshheading:8816705-Neural Cell Adhesion Molecules, pubmed-meshheading:8816705-Neural Inhibition, pubmed-meshheading:8816705-Neuronal Plasticity, pubmed-meshheading:8816705-Organ Culture Techniques, pubmed-meshheading:8816705-Rats, pubmed-meshheading:8816705-Rats, Sprague-Dawley, pubmed-meshheading:8816705-Receptors, N-Methyl-D-Aspartate, pubmed-meshheading:8816705-Synapses
pubmed:year
1996
pubmed:articleTitle
PSA-NCAM is required for activity-induced synaptic plasticity.
pubmed:affiliation
Department of Morphology Centre Médical Universitaire, Geneva, Switzerland.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't