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rdf:type |
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lifeskim:mentions |
umls-concept:C0033268,
umls-concept:C0035015,
umls-concept:C0085424,
umls-concept:C0123759,
umls-concept:C0205276,
umls-concept:C0301872,
umls-concept:C0596995,
umls-concept:C1292733,
umls-concept:C1515895,
umls-concept:C1548437,
umls-concept:C1711351,
umls-concept:C1882923
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pubmed:issue |
17
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pubmed:dateCreated |
1996-10-31
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pubmed:abstractText |
The p40 subunit of interleukin 12 (IL-12p40) has been known to act as an IL-12 antagonist in vitro. We here describe the immunosuppressive effect of IL-12p40 in vivo. A murine myoblast cell line, C2C12, was transduced with retro-virus vectors carrying the lacZ gene as a marker and the IL-12p40 gene. IL-12p40 secreted from the transfectant inhibited the IL-12-induced interferon gamma (IFN-gamma) production by splenocytes in vitro. Survival of C2C12 transplanted into allogeneic recipients was substantially prolonged when transduced with IL-12p40. Cytokine (IL-2 and IFN-gamma) production and cytotoxic T lymphocyte induction against allogeneic C2C12 were impaired in the recipients transplanted with the IL-12p40 transfectant. Delayed-type hypersensitivity response against C2C12 was also diminished in the IL-12p40 recipients. Furthermore, serum antibodies against beta-galactosidase of the T-helper 1-dependent isotypes (IgG2 and IgG3) were decreased in the IL-12p40 recipients. These results indicate that locally produced IL-12p40 exerts a potent immunosuppressive effect on T-helper 1-mediated immune responses that lead to allograft rejection. Therefore, IL-12p40 gene transduction would be useful for preventing the rejection of allografts and genetically modified own cells that are transduced with potentially antigenic molecules in gene therapy.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8799158-1350290,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8799158-1557125,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8799158-1584246,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8799158-1672545,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8799158-1673147,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8799158-1693082,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8799158-1713608,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8799158-1715362,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8799158-2204066,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8799158-2504877,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8799158-3042151,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8799158-563524,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8799158-7527811,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8799158-7528773,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8799158-7546194,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8799158-7638177,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8799158-7705395,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8799158-7705414,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8799158-7826531,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8799158-7843232,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8799158-7862626,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8799158-7877990,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8799158-7880387,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8799158-7907633,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8799158-7925572,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8799158-8096238,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8799158-8097319,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8799158-8103745,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8799158-8183921,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8799158-8355788,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8799158-8413366,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8799158-8620500
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
20
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pubmed:volume |
93
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
9085-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8799158-Animals,
pubmed-meshheading:8799158-Cytokines,
pubmed-meshheading:8799158-Graft Rejection,
pubmed-meshheading:8799158-Graft Survival,
pubmed-meshheading:8799158-Hematopoietic Stem Cell Transplantation,
pubmed-meshheading:8799158-Hypersensitivity, Delayed,
pubmed-meshheading:8799158-Immunoglobulin Isotypes,
pubmed-meshheading:8799158-Immunosuppression,
pubmed-meshheading:8799158-Interleukin-12,
pubmed-meshheading:8799158-Mice,
pubmed-meshheading:8799158-Mice, Inbred C57BL,
pubmed-meshheading:8799158-Recombinant Proteins,
pubmed-meshheading:8799158-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:8799158-Th1 Cells,
pubmed-meshheading:8799158-Transfection,
pubmed-meshheading:8799158-Transplantation, Homologous
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pubmed:year |
1996
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pubmed:articleTitle |
Local production of the p40 subunit of interleukin 12 suppresses T-helper 1-mediated immune responses and prevents allogeneic myoblast rejection.
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pubmed:affiliation |
Department of Immunology, Juntendo University School of Medicine, Tokyo, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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