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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
38
|
| pubmed:dateCreated |
1996-11-19
|
| pubmed:abstractText |
Platelets, essential for vascular integrity and hemostasis, fragment from polyploid megakaryocytes, characterized by their endomitotic cell cycle. We studied the influence of overexpression of c-myc oncogene on megakaryopoiesis and endomitosis in vivo, using transgenic mice carrying c-myc fused to the estrogen receptor under the control of the platelet factor 4 (PF4) megakaryocyte-specific promoter. The rationale behind this strategy was to obtain controlled overexpression of an active c-Myc, depending on the estrogen level in the mouse circulation. Analysis of these transgenic mice revealed that the bone marrow of female transgenic mice or of estrogen-injected male transgenic mice, but not of age-matched transgenic males nor nontransgenic females, contained frequent immature myeloid cells and an increased number of megakaryocytes. Deregulated expression of c-Myc shifted the normal ploidy profile of megakaryocytes due to a significant increase in proliferating megakaryocytes and a decrease in the fraction of ploidizing cells. These transgenic mice represent a novel in vivo model for a Myc-induced myeloproliferative disorder which can be controlled.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Estrogens,
http://linkedlifedata.com/resource/pubmed/chemical/Platelet Factor 4,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-myc,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Estrogen,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0021-9258
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
20
|
| pubmed:volume |
271
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
22976-82
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:8798484-Animals,
pubmed-meshheading:8798484-Bone Marrow,
pubmed-meshheading:8798484-Erythropoiesis,
pubmed-meshheading:8798484-Estrogens,
pubmed-meshheading:8798484-Female,
pubmed-meshheading:8798484-Gene Expression Regulation, Developmental,
pubmed-meshheading:8798484-Hematopoiesis,
pubmed-meshheading:8798484-Male,
pubmed-meshheading:8798484-Megakaryocytes,
pubmed-meshheading:8798484-Mice,
pubmed-meshheading:8798484-Mice, Transgenic,
pubmed-meshheading:8798484-Mitosis,
pubmed-meshheading:8798484-Myeloproliferative Disorders,
pubmed-meshheading:8798484-Platelet Factor 4,
pubmed-meshheading:8798484-Polyploidy,
pubmed-meshheading:8798484-Promoter Regions, Genetic,
pubmed-meshheading:8798484-Proto-Oncogene Proteins c-myc,
pubmed-meshheading:8798484-Receptors, Estrogen,
pubmed-meshheading:8798484-Recombinant Fusion Proteins,
pubmed-meshheading:8798484-Spleen,
pubmed-meshheading:8798484-Sternum
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
Deregulated expression of c-myc in megakaryocytes of transgenic mice increases megakaryopoiesis and decreases polyploidization.
|
| pubmed:affiliation |
Department of Biochemistry, Boston University School of Medicine, Boston, Massachusetts 02139, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|