Linked Life Data

8790393

Source:http://linkedlifedata.com/resource/pubmed/id/8790393

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
18
pubmed:dateCreated
1996-10-24
pubmed:abstractText
Vaccinia virus is a complex DNA virus that exhibits significant genetic and physical autonomy from the host cell. Most if not all of the functions involved in replication and transcription of the 192-kb genome are virally encoded. Although significant progress has been made in identifying trans-acting factors involved in DNA synthesis, the mechanism of genome replication has remained poorly understood. The genome is a linear duplex with covalently closed hairpin termini, and it has been presumed that sequences and/or structures within these termini are important for the initiation of genome replication. In this report we describe the construction of minichromosomes containing a central plasmid insert flanked by hairpin termini derived from the viral genome and their use as replication templates. When replication of these minichromosomes was compared with a control substrate containing synthetic hairpin termini, specificity for viral telomeres was apparent. Inclusion of > or = 200 bp from the viral telomere was sufficient to confer optimal replication efficiency, whereas 65-bp telomeres were not effective. Chimeric 200-bp telomeres containing the 65-bp terminal element and 135 bp of ectopic sequence also failed to confer efficient replication, providing additional evidence that telomere function is sequence-specific. Replication of these exogenous templates was dependent upon the viral replication machinery, was temporally coincident with viral replication, and generated covalently closed minichromosome products. These data provide compelling evidence for specificity in template recognition and utilization in vaccinia virus-infected cells.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8790393-1291243, http://linkedlifedata.com/resource/pubmed/commentcorrection/8790393-1845909, http://linkedlifedata.com/resource/pubmed/commentcorrection/8790393-1974828, http://linkedlifedata.com/resource/pubmed/commentcorrection/8790393-2197987, http://linkedlifedata.com/resource/pubmed/commentcorrection/8790393-2242682, http://linkedlifedata.com/resource/pubmed/commentcorrection/8790393-2242683, http://linkedlifedata.com/resource/pubmed/commentcorrection/8790393-2309453, http://linkedlifedata.com/resource/pubmed/commentcorrection/8790393-2911123, http://linkedlifedata.com/resource/pubmed/commentcorrection/8790393-3003742, http://linkedlifedata.com/resource/pubmed/commentcorrection/8790393-3016294, http://linkedlifedata.com/resource/pubmed/commentcorrection/8790393-3041037, http://linkedlifedata.com/resource/pubmed/commentcorrection/8790393-3085958, http://linkedlifedata.com/resource/pubmed/commentcorrection/8790393-6277506, http://linkedlifedata.com/resource/pubmed/commentcorrection/8790393-6322422, http://linkedlifedata.com/resource/pubmed/commentcorrection/8790393-6577746, http://linkedlifedata.com/resource/pubmed/commentcorrection/8790393-7060133, http://linkedlifedata.com/resource/pubmed/commentcorrection/8790393-7269237
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
3
pubmed:volume
93
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
9693-8
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Vaccinia virus DNA replication: two hundred base pairs of telomeric sequence confer optimal replication efficiency on minichromosome templates.
pubmed:affiliation
Graduate Program in Cell Biology and Genetics, Cornell University Graduate School of Medical Sciences, New York, NY, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't