Linked Life Data

8789147

Source:http://linkedlifedata.com/resource/pubmed/id/8789147

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1996-10-21
pubmed:abstractText
Pentafluorobenzyl chloroformate (PFBCF) has been utilized as a derivatization reagent for amino acids (AAs) in biological fluids with susequent detection by electron capture negative ionization mass spectrometry (ECNI/MS). AAs were derivatized in one step in aqueous solution, plasma, and whole blood at room temperature. To demonstrate quantitative analysis, phenylalanine concentrations were determined in human plasma. AAs were derivatized in one step using PFBCF and a mixture of water, ethanol, and pyridine/dimethylaminopyridine. The N-pentafluorobenzyloxycarbonyl amino acid ethyl esters (f phi-AA-OEt) exhibited good GC properties and the ECNI mass spectra are dominated by the [M-181]- ion. The f phi-AA-OEt derivatives can be easily detected at the femtomole level by selected ion monitoring. Phenethyl alcohol was also derivatized, using anhydrous conditions, and the resulting PFB carbonate's ECNI mass spectrum was dominated by the [M-181]- ion. The ECNI molar response of the PFB carbonate derivative is two times that of the corresponding pentafluorobenzoate.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0003-2697
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
233
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
58-66
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Analysis of amino acids in biological fluids by pentafluorobenzyl chloroformate derivatization and detection by electron capture negative ionization mass spectrometry.
pubmed:affiliation
Section on Analytical Biochemistry, National Institute of Mental Health, National Institutes of Health, Bethesda, Maryland 20892-1262, USA.
pubmed:publicationType
Journal Article