Linked Life Data

8774256

Source:http://linkedlifedata.com/resource/pubmed/id/8774256

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1996-10-2
pubmed:abstractText
1. Cirrhosis is often complicated by disturbances in the systemic circulation. We have previously demonstrated decreased vascular responses to vasoconstrictors in forearm resistance arteries in subjects with alcoholic cirrhosis. In the current study we investigate the role of the potent endogenous vasodilator nitric oxide in the peripheral circulation of these patients. 2. Ten patients with alcoholic cirrhosis (Pugh grade A) and 10 age-matched control subjects were studied. The effect of blockade of nitric oxide synthesis was studied both in vivo in forearm resistance arteries using forearm venous occlusion plethysmography and in vitro in veins isolated from the forearm. The role of endothelium-derived nitric oxide was studied in vivo using the endothelium-dependent vasodilator acetylcholine. 3. Mean arterial pressure and forearm basal flow in vivo were similar in the two groups. The constrictor response (percentage decrease in forearm blood flow) to noradrenaline (100 ng/min) was 26% smaller in patients with cirrhosis (31.65 +/- 2.64%) than in control subjects (42.75 +/- 3.87%, P = 0.037). Constrictor responses to the nitric oxide synthase inhibitor NG-monomethyl-L-arginine were not different in the two groups. Dilator responses to acetylcholine were significantly attenuated in cirrhotic patients compared with control subjects. 4. To investigate the role of smooth muscle-derived nitric oxide in vitro, all veins were stripped of their endothelium. Responses to noradrenaline were significantly diminished in veins isolated from patients with cirrhosis compared with control subjects. Incubation with the nitric oxide synthase inhibitor N omega-nitro-L-arginine had no effect on responses to noradrenaline in veins from control subjects but significantly enhanced the maximal response to noradrenaline by 23.95% (range 3.77-100%, P = 0.043) in veins from patients with cirrhosis. 5. Responses to noradrenaline were attenuated in vivo in forearm resistance arteries in patients with alcoholic cirrhosis. This impairment was also apparent in forearm isolated veins, stripped of the endothelium. Our data exclude a major role for endothelium-derived nitric oxide but highlight a possible role for smooth muscle-derived nitric oxide.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0143-5221
pubmed:author
pubmed:issnType
Print
pubmed:volume
91
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
23-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:8774256-Acetylcholine, pubmed-meshheading:8774256-Animals, pubmed-meshheading:8774256-Arginine, pubmed-meshheading:8774256-Dose-Response Relationship, Drug, pubmed-meshheading:8774256-Drug Interactions, pubmed-meshheading:8774256-Enzyme Inhibitors, pubmed-meshheading:8774256-Forearm, pubmed-meshheading:8774256-Humans, pubmed-meshheading:8774256-Liver Cirrhosis, Alcoholic, pubmed-meshheading:8774256-Male, pubmed-meshheading:8774256-Middle Aged, pubmed-meshheading:8774256-Muscle, Smooth, Vascular, pubmed-meshheading:8774256-Nitric Oxide, pubmed-meshheading:8774256-Norepinephrine, pubmed-meshheading:8774256-Plethysmography, pubmed-meshheading:8774256-Rats, pubmed-meshheading:8774256-Regional Blood Flow, pubmed-meshheading:8774256-Vascular Resistance, pubmed-meshheading:8774256-Veins, pubmed-meshheading:8774256-omega-N-Methylarginine
pubmed:year
1996
pubmed:articleTitle
Smooth muscle-derived nitric oxide is elevated in isolated forearm veins in human alcoholic cirrhosis.
pubmed:affiliation
Department of Gastroenterology, Baker Medical Research Institute, Melbourne, Victoria, Australia.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't