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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions |
umls-concept:C0001480,
umls-concept:C0024467,
umls-concept:C0024485,
umls-concept:C0086418,
umls-concept:C0178719,
umls-concept:C0242691,
umls-concept:C0242694,
umls-concept:C0330390,
umls-concept:C1552644,
umls-concept:C1553628,
umls-concept:C1705241,
umls-concept:C1705242,
umls-concept:C1720529,
umls-concept:C1823153,
umls-concept:C1880497,
umls-concept:C1948027,
umls-concept:C1948059,
umls-concept:C1996904,
umls-concept:C2349976,
umls-concept:C2603343
|
| pubmed:issue |
1
|
| pubmed:dateCreated |
1996-10-24
|
| pubmed:abstractText |
Localized proton decoupled 31P in vivo NMR spectroscopy of the human calf muscle was performed using a 1.5-T whole-body imager and the slice selective two-dimensional chemical-shift-imaging (2D-CSI) technique. The 31P-31P coupling constants and the chemical shifts of ATP were compared in gastrocnemius and soleus. Significant differences were found in the coupling constant J gamma beta: (18.1 +/- 0.7) Hz versus (17.1 +/- 0.6) Hz (means +/- SD, P < 10-5). Differences were also observed in the chemical shift separation delta alpha beta between the alpha- and beta-ATP signal: (8.498 +/- 0.023) ppm versus (8.522 +/- 0.222) ppm (p < 0.001) in gastrocnemius and soleus, respectively. A higher [MgATP]/[ATPfree] ratio and a significantly higher level of intracellular free magnesium of (0.52 +/- 0.06) mM in gastrocnemius versus (0.46 +/- 0.05) mM in soleus (p < 0.001) can be derived based on delta alpha beta and KDMgATP. Heterogeneity needs to be taken into account in clinical studies on magnesium by NMR methods in calf muscle. The coupling constant J gamma beta provides additional information, possibly on enzymatic processes, and correlates with [Mg2+free]. The detailed analysis of muscles with different fiber type characteristics lends support to the significance of this parameter in evaluating metabolism. The data reported can be used as prior knowledge for fits in which the coupling constants are set to a fixed value.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0968-5243
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
4
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
47-53
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8774001-Adenosine Triphosphate,
pubmed-meshheading:8774001-Adult,
pubmed-meshheading:8774001-Female,
pubmed-meshheading:8774001-Humans,
pubmed-meshheading:8774001-Intracellular Fluid,
pubmed-meshheading:8774001-Magnesium,
pubmed-meshheading:8774001-Magnetic Resonance Spectroscopy,
pubmed-meshheading:8774001-Male,
pubmed-meshheading:8774001-Middle Aged,
pubmed-meshheading:8774001-Muscle, Skeletal,
pubmed-meshheading:8774001-Phosphorus
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
31P NMR studies of human soleus and gastrocnemius show differences in the J gamma beta coupling constant of ATP and in intracellular free magnesium.
|
| pubmed:affiliation |
Hypertension and Diabetes Research Unit, Max Grundig Clinic, Bühl, Germany.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|