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rdf:type |
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lifeskim:mentions |
|
|
pubmed:issue |
6
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pubmed:dateCreated |
1996-9-25
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|
pubmed:abstractText |
Monoclonal antibodies specific to pokeweed antiviral protein (PAP), a ribosome-inactivating protein (RIP), were obtained after six unsuccessful fusions. A special procedure including injections of low doses of purified PAP from spring leaves in a short period was adopted. Some clones highly specific to PAP react with recombinant PAP. One clone cross-reacts with PAP-S isolated from seeds but none cross-reacts with the isoform PAP II isolated from summer leaves. These antibodies represent a useful tool to investigate the mechanisms of PAP biosynthesis and plant protection involving RIPs.
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pubmed:language |
eng
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pubmed:journal |
|
|
pubmed:citationSubset |
IM
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pubmed:chemical |
|
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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|
pubmed:issn |
0272-457X
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|
pubmed:author |
|
|
pubmed:issnType |
Print
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pubmed:volume |
14
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
571-5
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:8770645-Animals,
pubmed-meshheading:8770645-Antibodies, Monoclonal,
pubmed-meshheading:8770645-Antibody Specificity,
pubmed-meshheading:8770645-Hybridomas,
pubmed-meshheading:8770645-Immunization,
pubmed-meshheading:8770645-Mice,
pubmed-meshheading:8770645-Mice, Inbred BALB C,
pubmed-meshheading:8770645-N-Glycosyl Hydrolases,
pubmed-meshheading:8770645-Plant Proteins,
pubmed-meshheading:8770645-Protein Synthesis Inhibitors,
pubmed-meshheading:8770645-Ribosome Inactivating Proteins, Type 1
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|
pubmed:year |
1995
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|
pubmed:articleTitle |
Production and characterization of monoclonal antibodies against the ribosome-inactivating protein PAP from Phytolacca americana.
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|
pubmed:affiliation |
Laboratoire de Biochimie et Biologie Moléculaire, U.F.R. des Sciences et Techniques, Université de Franche Comté, Besançon, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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