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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1997-3-10
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pubmed:abstractText |
We synthesized a set of four biotinylated dideoxynucleoside triphosphates (biotin-9-ddNTPs) and optimized the reaction conditions for non-radioactive cycle sequencing using modified Tth DNA polymerase (delta Tth) and a chemiluminescent detection system. The resulting sequencing ladders showed lower background compared to those with the conventional non-radioactive sequencing method which uses 5'-biotinylated primers, especially when PCR products were analysed. With our method, DNA sequences can be determined at any primer positions without preparing 5'-biotinylated primers for dideoxy chain-termination.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
1340-2838
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
31
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pubmed:volume |
2
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
225-7
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:8770564-Biotin,
pubmed-meshheading:8770564-DNA Gyrase,
pubmed-meshheading:8770564-DNA Topoisomerases, Type II,
pubmed-meshheading:8770564-DNA-Directed DNA Polymerase,
pubmed-meshheading:8770564-Deoxyribonucleotides,
pubmed-meshheading:8770564-Luminescent Measurements,
pubmed-meshheading:8770564-Molecular Structure,
pubmed-meshheading:8770564-Sequence Analysis, DNA,
pubmed-meshheading:8770564-Thermus thermophilus
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pubmed:year |
1995
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pubmed:articleTitle |
A non-radioactive DNA sequencing method using biotinylated dideoxynucleoside triphosphates and delta Tth DNA polymerase.
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pubmed:affiliation |
Tsuruga Institute of Biotechnology, Toyobo Co., Ltd., Fukui, Japan.
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pubmed:publicationType |
Journal Article
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