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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1996-9-25
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pubmed:abstractText |
In isolated rabbit proximal tubules the addition of 2.0 microM but not 0.2 microM ionomycin induced a sustained increase in cell pH ([pH]i). This [pH]i response to 2.0 microM ionomycin was shown to be independent of several transporters such as Na+/H+ exchanger, Na(+)-HCO3- cotransporter, Cl-/HCO3- exchanger, or H(+)-ATPase. On the other hand, the removal of extracellular Ca2+ abolished the [pH]i increase or even induced a transient [pH]i decrease in the presence of ionomycin. These results are consistent with the induction of Ca2+/H+ exchange by ionomycin. Therefore Ca2+ ionophores should be used with caution as probes to estimate renal tubule functions.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
5
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pubmed:volume |
225
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
215-8
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8769120-Animals,
pubmed-meshheading:8769120-Bicarbonates,
pubmed-meshheading:8769120-Calcium,
pubmed-meshheading:8769120-Carrier Proteins,
pubmed-meshheading:8769120-Female,
pubmed-meshheading:8769120-Hydrogen-Ion Concentration,
pubmed-meshheading:8769120-Ionomycin,
pubmed-meshheading:8769120-Kidney Tubules, Proximal,
pubmed-meshheading:8769120-Kinetics,
pubmed-meshheading:8769120-Proton-Translocating ATPases,
pubmed-meshheading:8769120-Rabbits,
pubmed-meshheading:8769120-Time Factors
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pubmed:year |
1996
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pubmed:articleTitle |
Effect of ionomycin on cell pH in isolated renal proximal tubules.
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pubmed:affiliation |
First Department of Internal Medicine, Tokyo University School of Medicine, Japan.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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