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rdf:type |
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lifeskim:mentions |
umls-concept:C0007634,
umls-concept:C0022646,
umls-concept:C0022677,
umls-concept:C0033684,
umls-concept:C0205410,
umls-concept:C0205460,
umls-concept:C0218146,
umls-concept:C0728938,
umls-concept:C0871261,
umls-concept:C1704632,
umls-concept:C1706817,
umls-concept:C1948027,
umls-concept:C2003941,
umls-concept:C2911692
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pubmed:issue |
1
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pubmed:dateCreated |
1996-9-25
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pubmed:abstractText |
Peptide YY (PYY)-preferring receptors are expressed in the renal proximal tubule cell clone Cl.10 isolated from the PKSV-PCT cell line. They mediate PYY-inhibited cAMP production through coupling with pertussis-sensitive Gi proteins. Previous G alpha i RNA antisense experiments demonstrated the exclusive coupling of the PYY receptor to the Gi2 protein. Here we characterized a clone stably expressing G alpha i2 antisense RNA which exhibited only a partial decrease in G alpha i2 content (#60%) as estimated by Western blot. When compared to control Cl.10 cells, this clone, referred to as Cl.10(t), exhibited: (i) an increase in the dissociation constant of PYY receptors (6.42 vs 0.63 nM); (ii) a complete absence of inhibition of [125I]PYY binding by GTP gamma S and GTP; (iii) the failure of PYY to inhibit basal and forskolin-stimulated cAMP levels; (iv) the failure of PYY to stimulate [35S]GTP gamma S binding to membranes. These findings show that partial knockdown of G alpha i2 expression in Cl.10 cells completely abolish the coupling of PYY receptors to biological response.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Guanosine 5'-O-(3-Thiotriphosphate),
http://linkedlifedata.com/resource/pubmed/chemical/Guanosine Triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Macromolecular Substances,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotides, Antisense,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide YY,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Gastrointestinal Hormone,
http://linkedlifedata.com/resource/pubmed/chemical/peptide YY receptor
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
5
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pubmed:volume |
225
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
16-21
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8769088-Animals,
pubmed-meshheading:8769088-Base Sequence,
pubmed-meshheading:8769088-Cell Membrane,
pubmed-meshheading:8769088-Clone Cells,
pubmed-meshheading:8769088-Cyclic AMP,
pubmed-meshheading:8769088-GTP-Binding Proteins,
pubmed-meshheading:8769088-Guanosine 5'-O-(3-Thiotriphosphate),
pubmed-meshheading:8769088-Guanosine Triphosphate,
pubmed-meshheading:8769088-Immunoblotting,
pubmed-meshheading:8769088-Kidney Tubules, Proximal,
pubmed-meshheading:8769088-Kinetics,
pubmed-meshheading:8769088-Macromolecular Substances,
pubmed-meshheading:8769088-Molecular Sequence Data,
pubmed-meshheading:8769088-Oligonucleotides, Antisense,
pubmed-meshheading:8769088-Peptide YY,
pubmed-meshheading:8769088-Peptides,
pubmed-meshheading:8769088-Receptors, Gastrointestinal Hormone,
pubmed-meshheading:8769088-Swine,
pubmed-meshheading:8769088-Transfection
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pubmed:year |
1996
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pubmed:articleTitle |
Partial knockdown of G alpha i2 protein is sufficient to abolish the coupling of PYY receptors to biological response in renal proximal tubule cells.
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pubmed:affiliation |
Unité de Neuroendocrinologie et Biologie Cellulaire Digestives, Institut National de la Santé et de la Recherche Médicale, INSERM U410, Faculté de Médecine X. Bichat, Paris, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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