Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
18-19
pubmed:dateCreated
1996-10-29
pubmed:abstractText
In the coming years, the accurate and rapid microbiological detection of mycobacteria will gain in importance, since infections with Mycobacterium tuberculosis and ubiquitous mycobacteria are expected to increase, in particular in immunocompromised persons. In addition to the classical biochemical methods of detection involving the use of the microscope and cultures, molecular biological techniques are already being used today. The present overview therefore discusses the principles of the major methods. These include the use of the major DNA probes, DNA amplification techniques (PCR) with subsequent DNA sequencing for species determination, and RFLP analysis, which is employed to deal with epidemiological problems. Also described is the Bactec method for culturing in a liquid medium which forms the basis of various modern examination techniques.
pubmed:language
ger
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0015-8178
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
114
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
237-41
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
[Molecular biology methods in detection of mycobacteria. Improved and newly developed test systems for the diagnosis of mycobacteria].
pubmed:affiliation
Institut für Medizinische Mikrobiologie und Hygiene, Universitätsklinikum Regensburg.
pubmed:publicationType
Journal Article, English Abstract, Review