Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1996-9-26
pubmed:abstractText
Lipoprotein lipase (LPL) has been implicated in the delivery of chylomicron-located alpha-tocopherol (alpha-TocH) to peripheral tissues. To investigate the role of LPL in the cellular uptake of alpha-TocH in peripheral tissue in vivo, three lines of transgenic mice [mouse creatine kinase- (MCK) L, MCK-M and MCK-H] expressing various amounts of human LPL were compared with regard to alpha-TocH levels in plasma, skeletal muscle, cardiac muscle, adipose tissue and brain. Depending on the copy number of the transgene, LPL activity was increased 3- to 27-fold in skeletal muscle and 1.3- to 3.7-fold in cardiac muscle. The intracellular levels of alpha-TocH in skeletal muscle were significantly increased in MCK-M and MCK-H animals and correlated highly with the tissue-specific LPL activity (r = 0.998). The highest levels were observed in MCK-H (21.4 nmol/g) followed by MCK-M (13.3 nmol/g) and MCK-L (8.2 nmol/g) animals when compared with control mice (7.3 nmol/g). Excellent correlation was also observed between intracellular alpha-TocH and non-esterified fatty acid (NEFA) levels (r = 0.998). Although LPL activities in cardiac muscle were also increased in the transgenic mouse lines, alpha-TocH concentrations in the heart remained unchanged. Similarly, alpha-TocH levels in plasma, adipose tissue and brain were unaffected by the tissue specific over-expression of LPL in muscle. The transgenic model presented in this report provides evidence that the uptake of alpha-TocH in muscle is directly dependent on the level of LPL expression in vivo. Increased intracellular alpha-TocH concentrations with increased triglyceride lipolysis and NEFA uptake might protect the myocyte from oxidative damage during increased beta-oxidation.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8761445-1320015, http://linkedlifedata.com/resource/pubmed/commentcorrection/8761445-1329730, http://linkedlifedata.com/resource/pubmed/commentcorrection/8761445-1334030, http://linkedlifedata.com/resource/pubmed/commentcorrection/8761445-13671378, http://linkedlifedata.com/resource/pubmed/commentcorrection/8761445-1401083, http://linkedlifedata.com/resource/pubmed/commentcorrection/8761445-1430223, http://linkedlifedata.com/resource/pubmed/commentcorrection/8761445-1581846, http://linkedlifedata.com/resource/pubmed/commentcorrection/8761445-1656440, http://linkedlifedata.com/resource/pubmed/commentcorrection/8761445-1838668, http://linkedlifedata.com/resource/pubmed/commentcorrection/8761445-2312476, http://linkedlifedata.com/resource/pubmed/commentcorrection/8761445-2340307, http://linkedlifedata.com/resource/pubmed/commentcorrection/8761445-2351872, http://linkedlifedata.com/resource/pubmed/commentcorrection/8761445-3311418, http://linkedlifedata.com/resource/pubmed/commentcorrection/8761445-3343344, http://linkedlifedata.com/resource/pubmed/commentcorrection/8761445-3790578, http://linkedlifedata.com/resource/pubmed/commentcorrection/8761445-3998153, http://linkedlifedata.com/resource/pubmed/commentcorrection/8761445-4019686, http://linkedlifedata.com/resource/pubmed/commentcorrection/8761445-5447010, http://linkedlifedata.com/resource/pubmed/commentcorrection/8761445-6263247, http://linkedlifedata.com/resource/pubmed/commentcorrection/8761445-6466698, http://linkedlifedata.com/resource/pubmed/commentcorrection/8761445-6486081, http://linkedlifedata.com/resource/pubmed/commentcorrection/8761445-6720939, http://linkedlifedata.com/resource/pubmed/commentcorrection/8761445-6959572, http://linkedlifedata.com/resource/pubmed/commentcorrection/8761445-6996570, http://linkedlifedata.com/resource/pubmed/commentcorrection/8761445-7510694, http://linkedlifedata.com/resource/pubmed/commentcorrection/8761445-7635990, http://linkedlifedata.com/resource/pubmed/commentcorrection/8761445-7666010, http://linkedlifedata.com/resource/pubmed/commentcorrection/8761445-7719340, http://linkedlifedata.com/resource/pubmed/commentcorrection/8761445-7766695, http://linkedlifedata.com/resource/pubmed/commentcorrection/8761445-7797576, http://linkedlifedata.com/resource/pubmed/commentcorrection/8761445-7887897, http://linkedlifedata.com/resource/pubmed/commentcorrection/8761445-7922298, http://linkedlifedata.com/resource/pubmed/commentcorrection/8761445-8005519, http://linkedlifedata.com/resource/pubmed/commentcorrection/8761445-8015482, http://linkedlifedata.com/resource/pubmed/commentcorrection/8761445-8206972, http://linkedlifedata.com/resource/pubmed/commentcorrection/8761445-8250868, http://linkedlifedata.com/resource/pubmed/commentcorrection/8761445-8300427, http://linkedlifedata.com/resource/pubmed/commentcorrection/8761445-8429255, http://linkedlifedata.com/resource/pubmed/commentcorrection/8761445-8487628, http://linkedlifedata.com/resource/pubmed/commentcorrection/8761445-8582456, http://linkedlifedata.com/resource/pubmed/commentcorrection/8761445-8656074, http://linkedlifedata.com/resource/pubmed/commentcorrection/8761445-870071
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0264-6021
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
318 ( Pt 1)
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
15-9
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Muscle-specific overexpression of lipoprotein lipase in transgenic mice results in increased alpha-tocopherol levels in skeletal muscle.
pubmed:affiliation
Department of Medical Biochemistry, University of Graz, Austria.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't