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pubmed-article:8750651pubmed:abstractTextThe ultrasensitive PSA assay has been recently acknowledged as a useful tool for the monitoring of patients prostatectomized for prostatic cancer. We have evaluated a commercially available ultrasensitive PSA assay (Immulite Third Generation PSA-DPC-Los Angeles CA) in comparison with the routinely used PSA (Immulite PSA-DPC-Los Angeles CA). When evaluated with different approaches, the analytical sensitivity of ultrasensitive PSA ranged between 0.0029 and 0.0038 ng/ml. The biological detection limit was 0.0098 ng/ml. Dilution of samples with low PSA levels showed a good recovery (from 88 to 113%) up to 1:128 dilution factor (final PSA levels ranging from 0.004 to 0.016 ng/ml in different samples). The assay precision was excellent in the low dose range, the highest interassay interadjustment CV among replicates being 5.84% when assaying serum samples with PSA lower than 1.0 ng/ml. Besides its role in the follow-up of prostatectomized patients, the evaluated ultrasensitive PSA could be reliably used for the detection of clinically meaningful PSA variations in the low dose range, and it could therefore be a candidate for the assessment of PSA velocity.lld:pubmed
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pubmed-article:8750651pubmed:pagination229-33lld:pubmed
pubmed-article:8750651pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:8750651pubmed:articleTitleThird-generation PSA: ultrasensitive or ultraprecise assay?lld:pubmed
pubmed-article:8750651pubmed:affiliationCenter for the Study of Biological Markers of Malignancy, National Center for the Application of Biotechnologies in Oncology, Venezia, Italy.lld:pubmed
pubmed-article:8750651pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:8750651pubmed:publicationTypeComparative Studylld:pubmed