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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1997-3-11
|
| pubmed:abstractText |
Clinical isolates of Yersinia enterocolitca, which belong to mouse-lethal serotypes, produce the siderophore yersiniophore. Siderophore production was shown to be iron regulated and to reach maximum production in late log phase. Yersiniophore is a fluorescent siderophore with maximum excitation at 270 nm and a major emission peak at 428 nm. Absorption maxima were seen at 210 and 250 nm with a low broad peak from 280 to 320 nm. Purification of unchelated yersiniophore for structural analysis was made difficult by low yields (1-2 mg mg-1), and susceptibility to acid hydrolysis, oxidation and possibly polymerization. Yersinophore was therefore purified as an Al3+ chelate, which was found to be stable in solution for several weeks. To purify Al(3+)-yersinophore, unchelated yersiniophore was first extracted from culture supernatants with dichloromethane, concentrated by rotary evaporation and adsorbed to a DEAE-sephacel column. Al(3+)-yersiniophore was eluted with 0.01 M AlCl3 and further purified by HPLC. The structure was established by a combination of elemental analysis, high resolution mass spectrometry and two-dimensional NMR experiments. Yersiniophore is a phenolate-thiazole siderophore with the formula C21H24N3O4S3Al and a molecular weight of 505.07404 when chelated to Al3+. The structure of yersiniophore was determined to be closely related to the structures of pyochelin, produced by Pseudomonas aeruginosa, and anguibactin, produced by Vibrio anguillarum.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Aluminum,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/Phenols,
http://linkedlifedata.com/resource/pubmed/chemical/Siderophores,
http://linkedlifedata.com/resource/pubmed/chemical/Thiazoles,
http://linkedlifedata.com/resource/pubmed/chemical/anguibactin,
http://linkedlifedata.com/resource/pubmed/chemical/pyochelin
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
0966-0844
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
9
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
157-67
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8744899-Aluminum,
pubmed-meshheading:8744899-Animals,
pubmed-meshheading:8744899-Chromatography, High Pressure Liquid,
pubmed-meshheading:8744899-Chromatography, Ion Exchange,
pubmed-meshheading:8744899-Feces,
pubmed-meshheading:8744899-Humans,
pubmed-meshheading:8744899-Magnetic Resonance Spectroscopy,
pubmed-meshheading:8744899-Mass Spectrometry,
pubmed-meshheading:8744899-Mice,
pubmed-meshheading:8744899-Peptides,
pubmed-meshheading:8744899-Phenols,
pubmed-meshheading:8744899-Pseudomonas aeruginosa,
pubmed-meshheading:8744899-Siderophores,
pubmed-meshheading:8744899-Spectrometry, Fluorescence,
pubmed-meshheading:8744899-Spectrophotometry, Ultraviolet,
pubmed-meshheading:8744899-Structure-Activity Relationship,
pubmed-meshheading:8744899-Thiazoles,
pubmed-meshheading:8744899-Vibrio,
pubmed-meshheading:8744899-Yersinia enterocolitica
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
Physical and structural characterization of yersiniophore, a siderophore produced by clinical isolates of Yersinia enterocolitica.
|
| pubmed:affiliation |
Department of Microbiology, University of Calgary, Alberta, Canada.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|