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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1996-12-10
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pubmed:databankReference | |
pubmed:abstractText |
The sigma B transcription factor of Bacillus subtilis is activated in response to a variety of environmental stresses, including those imposed by entry into the stationary-growth phase, and by heat, salt or ethanol challenge to logarithmically growing cells. Although sigma B is thought to control a general stress regulon, the range of cellular functions it directs remains largely unknown. Our approach to understand the physiological role of sigma B is to characterize genes that require this factor for all or part of their expression, i.e. the csb genes. In this study, we report that the transposon insertion csb40::Tn917lac identifies an operon with three open reading frames, the second of which resembles plant proteins induced by desiccation stress. Primer-extension and operon-fusion experiments showed that the csb40 operon has a sigma B-dependent promoter which is strongly induced by the addition of salt to logarithmically growing cells. The csb40 operon also has a second, sigma H-dependent promoter that is unaffected by salt addition. These results provide support for the hypothesis that sigma B controls a general stress regulon, and indicate that the sigma B and sigma H regulons partly overlap. We suggest that in addition to its acknowledged role in the sporulation process, sigma H is also involved in controlling a subclass of genes that are broadly involved in a general stress response.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/SigB protein, Bacteria,
http://linkedlifedata.com/resource/pubmed/chemical/Sigma Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Spo0H protein, Bacillus subtilis,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0950-382X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
20
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
339-50
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:8733232-Amino Acid Sequence,
pubmed-meshheading:8733232-Bacillus subtilis,
pubmed-meshheading:8733232-Bacterial Proteins,
pubmed-meshheading:8733232-Base Sequence,
pubmed-meshheading:8733232-Cloning, Molecular,
pubmed-meshheading:8733232-DNA, Bacterial,
pubmed-meshheading:8733232-Gene Expression Regulation, Bacterial,
pubmed-meshheading:8733232-Molecular Sequence Data,
pubmed-meshheading:8733232-Mutagenesis, Insertional,
pubmed-meshheading:8733232-Open Reading Frames,
pubmed-meshheading:8733232-Operon,
pubmed-meshheading:8733232-Phenotype,
pubmed-meshheading:8733232-Promoter Regions, Genetic,
pubmed-meshheading:8733232-Sigma Factor,
pubmed-meshheading:8733232-Spores, Bacterial,
pubmed-meshheading:8733232-Transcription Factors
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pubmed:year |
1996
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pubmed:articleTitle |
Bacillus subtilis operon under the dual control of the general stress transcription factor sigma B and the sporulation transcription factor sigma H.
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pubmed:affiliation |
Department of Food Science and Technology, University of California, Davis 95616, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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