| pubmed-article:8732167 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8732167 | lifeskim:mentions | umls-concept:C0017296 | lld:lifeskim |
| pubmed-article:8732167 | lifeskim:mentions | umls-concept:C1337112 | lld:lifeskim |
| pubmed-article:8732167 | lifeskim:mentions | umls-concept:C0679622 | lld:lifeskim |
| pubmed-article:8732167 | lifeskim:mentions | umls-concept:C1707689 | lld:lifeskim |
| pubmed-article:8732167 | lifeskim:mentions | umls-concept:C0205314 | lld:lifeskim |
| pubmed-article:8732167 | lifeskim:mentions | umls-concept:C1514152 | lld:lifeskim |
| pubmed-article:8732167 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:8732167 | pubmed:dateCreated | 1996-10-24 | lld:pubmed |
| pubmed-article:8732167 | pubmed:abstractText | The promise of effective gene therapy can only be accomplished by high-level expression and regulatable delivery of gene products. To achieve this end, a eukaryotic expression plasmid was modified to make transcription dependent on a tetracycline(Tc)-regulated chimeric transactivator. Mouse muscle injected with this two plasmid cis/trans control system expressed reporter proteins at levels five- to 10-fold greater than the cytomegalovirus immediate-early promoter-controlled parental plasmid. Tetracycline could be useful to either repress or activate transactivator-controlled expression based on the position of the tetO control sequences within the reporter plasmid. Finally, a prototype single plasmid construct was made and shown to express a self-regulating bicistronic transcript containing both the reporter and the transactivator. These Tc-controlled plasmids, termed maximum expression and regulated vectors (MERVs), have the potential to target a variety of gene therapy applications. | lld:pubmed |
| pubmed-article:8732167 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8732167 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8732167 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8732167 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8732167 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8732167 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8732167 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8732167 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8732167 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8732167 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8732167 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:8732167 | pubmed:issn | 0969-7128 | lld:pubmed |
| pubmed-article:8732167 | pubmed:author | pubmed-author:ManthorpeMM | lld:pubmed |
| pubmed-article:8732167 | pubmed:author | pubmed-author:HobartPP | lld:pubmed |
| pubmed-article:8732167 | pubmed:author | pubmed-author:HartikkaJJ | lld:pubmed |
| pubmed-article:8732167 | pubmed:author | pubmed-author:LiangXX | lld:pubmed |
| pubmed-article:8732167 | pubmed:author | pubmed-author:SukhuLL | lld:pubmed |
| pubmed-article:8732167 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8732167 | pubmed:volume | 3 | lld:pubmed |
| pubmed-article:8732167 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8732167 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8732167 | pubmed:pagination | 350-6 | lld:pubmed |
| pubmed-article:8732167 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
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| pubmed-article:8732167 | pubmed:meshHeading | pubmed-meshheading:8732167-... | lld:pubmed |
| pubmed-article:8732167 | pubmed:year | 1996 | lld:pubmed |
| pubmed-article:8732167 | pubmed:articleTitle | Novel, high expressing and antibiotic-controlled plasmid vectors designed for use in gene therapy. | lld:pubmed |
| pubmed-article:8732167 | pubmed:affiliation | Department of Molecular Biology, Vical Incorporated, San Diego, CA 92121, USA. | lld:pubmed |
| pubmed-article:8732167 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8732167 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8732167 | lld:pubmed |
