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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1996-10-24
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pubmed:abstractText |
The promise of effective gene therapy can only be accomplished by high-level expression and regulatable delivery of gene products. To achieve this end, a eukaryotic expression plasmid was modified to make transcription dependent on a tetracycline(Tc)-regulated chimeric transactivator. Mouse muscle injected with this two plasmid cis/trans control system expressed reporter proteins at levels five- to 10-fold greater than the cytomegalovirus immediate-early promoter-controlled parental plasmid. Tetracycline could be useful to either repress or activate transactivator-controlled expression based on the position of the tetO control sequences within the reporter plasmid. Finally, a prototype single plasmid construct was made and shown to express a self-regulating bicistronic transcript containing both the reporter and the transactivator. These Tc-controlled plasmids, termed maximum expression and regulated vectors (MERVs), have the potential to target a variety of gene therapy applications.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0969-7128
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
3
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
350-6
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:8732167-Animals,
pubmed-meshheading:8732167-Anti-Bacterial Agents,
pubmed-meshheading:8732167-Base Sequence,
pubmed-meshheading:8732167-Cell Line,
pubmed-meshheading:8732167-Chloramphenicol O-Acetyltransferase,
pubmed-meshheading:8732167-Cytomegalovirus,
pubmed-meshheading:8732167-DNA, Recombinant,
pubmed-meshheading:8732167-Gene Expression,
pubmed-meshheading:8732167-Gene Therapy,
pubmed-meshheading:8732167-Genetic Vectors,
pubmed-meshheading:8732167-Humans,
pubmed-meshheading:8732167-Mice,
pubmed-meshheading:8732167-Molecular Sequence Data,
pubmed-meshheading:8732167-Muscles,
pubmed-meshheading:8732167-Plasmids,
pubmed-meshheading:8732167-Recombinant Fusion Proteins,
pubmed-meshheading:8732167-Tetracycline,
pubmed-meshheading:8732167-Trans-Activators,
pubmed-meshheading:8732167-Transcriptional Activation
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pubmed:year |
1996
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pubmed:articleTitle |
Novel, high expressing and antibiotic-controlled plasmid vectors designed for use in gene therapy.
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pubmed:affiliation |
Department of Molecular Biology, Vical Incorporated, San Diego, CA 92121, USA.
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pubmed:publicationType |
Journal Article
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