Linked Life Data

8730584

Source:http://linkedlifedata.com/resource/pubmed/id/8730584

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1997-2-21
pubmed:abstractText
1. The influence of divalent cations was investigated on the noradrenaline-activated non-selective cation current (Icat) in freshly dispersed smooth muscle cells of rabbit portal vein. 2. Reduction of external calcium concentration ([Ca2+]o) from 1.5 mM to 'test' levels of 100-500 microM during stimulation of Icat produced a sustained potentiation of Icat, which indicates an inhibitory action of Cao2+. With 'test' concentrations lower than 100 microM Cao2+ the amplitude of Icat was initially potentiated and then the current subsequently declined. Under these conditions re-addition of 1.5 mM Cao2+ transiently activated Icat which demonstrates an additional facilitatory action of [Ca2+]o. The half-maximal [Ca2+]o for the facilitatory and inhibitory action on Icat was 6 and 400 microM, respectively. 3. When [Ca2+]o was decreased to very low levels (< 10 nM) by inclusion of 1 mM BAPTA in nominally Ca(2+)-free external solution, Icat could still be evoked by noradrenaline. This suggested that Ca2+ is not obligatory for activation of Icat but merely modulatory. 4. In 1.5 mM Cao2+ the current-voltage (I-V) relationship of Icat was S-shaped with a reversal potential (Er) of about +9 mV. The I-V characteristics and Er of the potentiated current in 200 microM Cao2+ were not markedly different from those in 1.5 mM Cao2+. 5. During the noradrenaline-induced Icat, removal of external Mg2+ caused a small increase in the amplitude of Icat which was not as great as that seen on reduction of [Ca2+]o and there was no facilitatory effect of Mgo2+. 6. These results indicate that external Ca2+ has a dual action on Icat, both facilitating and inhibiting the current. This dual action is not shared with external Mg2+.
pubmed:grant
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0022-3751
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
492 ( Pt 1)
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
75-88
pubmed:dateRevised
2010-8-25
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Dual effect of external Ca2+ on noradrenaline-activated cation current in rabbit portal vein smooth muscle cells.
pubmed:affiliation
Department of Pharmacology, St George's Hospital Medical School, London, UK.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't