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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6
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pubmed:dateCreated |
1996-11-27
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pubmed:abstractText |
The sperm plasma membrane protein PH-20 has a hyaluronidase activity that enables acrosome-intact sperm to pass through the cumulus cell layer of the egg. In this study we analyzed the relationship of guinea pig PH-20 and the "classical" soluble hyaluronidase released at the time of the acrosome reaction of guinea pig sperm. PH-20 is a membrane protein, anchored in the plasma and inner acrosomal membranes by a glycosyl phosphatidyl inositol anchor. Several types of experiments indicate a structural relationship of PH-20 and the soluble hyaluronidase released during the acrosome reaction. First, an antiserum raised against purified PH-20 is positive in an immunoblot of the soluble protein fraction released during the acrosome reaction. In the released, soluble protein fraction, the anti-PH-20 antiserum recognizes a protein of approximately 64 kDa, i.e., identical in molecular mass to PH-20 (approximately 64 kDa). Second, the enzymatic activity of the released hyaluronidase is completely inhibited (100%) by the anti-PH-20 antiserum. Third, almost all (97%) of the soluble hyaluronidase is removed from the released protein fraction by a single pass through an affinity column made with an anti-PH-20 monoclonal antibody. These findings suggest that the released, soluble hyaluronidase is a soluble form of PH-20 (sPH-20). During the acrosome reaction, PH-20 undergoes endoproteolytic cleavage into two disulfide-linked fragments whereas the released sPH-20 is not cleaved, suggesting the possible activity of a membrane-bound endoprotease on PH-20. We searched for a cDNA encoding sPH-20 but none was found. This result suggests that sPH-20 may arise from the enzymatic release of PH-20 from its membrane anchor, possibly at the time of acrosome reaction.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Adhesion Molecules,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Hyaluronoglucosaminidase,
http://linkedlifedata.com/resource/pubmed/chemical/hyaluronidase PH-20
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0006-3363
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
54
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1343-9
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pubmed:dateRevised |
2010-4-1
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pubmed:meshHeading |
pubmed-meshheading:8724363-Acrosome,
pubmed-meshheading:8724363-Animals,
pubmed-meshheading:8724363-Antibodies, Monoclonal,
pubmed-meshheading:8724363-Cell Adhesion Molecules,
pubmed-meshheading:8724363-Cell Membrane,
pubmed-meshheading:8724363-DNA, Complementary,
pubmed-meshheading:8724363-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:8724363-Guinea Pigs,
pubmed-meshheading:8724363-Hyaluronoglucosaminidase,
pubmed-meshheading:8724363-Hydrogen-Ion Concentration,
pubmed-meshheading:8724363-Immunoblotting,
pubmed-meshheading:8724363-Male,
pubmed-meshheading:8724363-Spermatozoa
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pubmed:year |
1996
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pubmed:articleTitle |
Structural relationship of sperm soluble hyaluronidase to the sperm membrane protein PH-20.
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pubmed:affiliation |
Department of Physiology, University of Connecticut Health Center, Farmington 06030, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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