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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1996-9-26
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pubmed:abstractText |
Astrocytes actively maintain their intracellular pH (pHi) more alkaline than expected by passive distribution of H+. Acid extruding transporters such as the amiloride-sensitive Na+/H+ exchanger (NHE) are necessary for pH regulation. Currently, four mammalian NHEs (NHE1-NHE4) have been cloned, with a fifth (NHE5) partially cloned. We attempted to determine which isoform(s) of NHE was present in cultured hippocampal astrocytes using amiloride sensitivity and immunospecificity as criteria. In the absence of HCO3-, amiloride blocked pHi recovery after an acid load with an IC50 of approximately 3.18 microM, similar to values reported for the amiloride-sensitive isoforms NHE1 and NHE2. Immunoblotting with a highly specific antibody for NHE1 identified a 100 kDa protein, indicating the presence of NHE1 in whole brain, hippocampus, and cultured hippocampal astrocytes. Further probing for an additional amiloride-sensitive NHE failed to detect evidence of the presence of NHE4. Surprisingly, application of the potent analog of amiloride, ethylisopropylamiloride (EIPA), caused a reversible alkalinization of pHi, suggesting the presence of an additional acid/base transport mechanism that is EIPA-sensitive.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Amiloride,
http://linkedlifedata.com/resource/pubmed/chemical/Bicarbonates,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium-Hydrogen Antiporter,
http://linkedlifedata.com/resource/pubmed/chemical/ethylisopropylamiloride,
http://linkedlifedata.com/resource/pubmed/chemical/growth factor-activatable Na-H...
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0360-4012
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
44
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
191-8
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8723228-Amiloride,
pubmed-meshheading:8723228-Animals,
pubmed-meshheading:8723228-Animals, Newborn,
pubmed-meshheading:8723228-Astrocytes,
pubmed-meshheading:8723228-Bicarbonates,
pubmed-meshheading:8723228-Cell Membrane,
pubmed-meshheading:8723228-Cells, Cultured,
pubmed-meshheading:8723228-Gene Expression,
pubmed-meshheading:8723228-Hippocampus,
pubmed-meshheading:8723228-Hydrogen-Ion Concentration,
pubmed-meshheading:8723228-Rats,
pubmed-meshheading:8723228-Rats, Sprague-Dawley,
pubmed-meshheading:8723228-Sodium-Hydrogen Antiporter
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pubmed:year |
1996
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pubmed:articleTitle |
Characterization of Na+/H+ exchange activity in cultured rat hippocampal astrocytes.
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pubmed:affiliation |
Department of Surgery (Neurosurgery), Yale University School of Medicine, New Haven, Connecticut 06510, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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