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pubmed-article:8719207pubmed:dateCreated1996-10-24lld:pubmed
pubmed-article:8719207pubmed:abstractTextThe head-associated lymphoid tissues of the chicken, composed of the harderian gland and the conjunctiva-associated lymphoid tissue (CALT), were studied to determine whether changes occurred in lymphocyte subpopulations as chickens age from 1 week to 8 weeks. The B cells and subpopulations of T-lymphocytes of the head-associated lymphoid tissues were identified using in situ immunohistochemical staining. Monoclonal antibodies specific for various lymphocyte surface antigens were used. The concentration of T-lymphocytes, particularly CD3+ and CD4+ cells, within the harderian gland increased with age, whereas the concentration of B cells remained the same. B-lymphocytes were observed within the germinal centers of the CALT of 4-week-old birds. The T-lymphocytes within the CALT surrounded the B-cell-rich germinal centers. CD3+ T-lymphocytes were the predominant cell population in all age groups examined. Increasing concentrations of CD3+, CD4+, and CD8+ T-lymphocytes were observed within the CALT as chicks developed from 1 week to 4 weeks of age. Finally, no changes were observed in lymphocyte populations within the CALT as chicks developed from 4 weeks to 8 weeks of age.lld:pubmed
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pubmed-article:8719207pubmed:authorpubmed-author:ReynoldsD LDLlld:pubmed
pubmed-article:8719207pubmed:authorpubmed-author:MaslakD MDMlld:pubmed
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pubmed-article:8719207pubmed:dateRevised2003-11-14lld:pubmed
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pubmed-article:8719207pubmed:articleTitleB cells and T-lymphocyte subsets of the head-associated lymphoid tissues of the chicken.lld:pubmed
pubmed-article:8719207pubmed:affiliationDepartment of Microbiology, Immunology, and Preventive Medicine, Iowa State University, Ames 50011, USA.lld:pubmed
pubmed-article:8719207pubmed:publicationTypeJournal Articlelld:pubmed