rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1 Spec No
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pubmed:dateCreated |
1996-9-11
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pubmed:abstractText |
A genetic fusion between the gene encoding green fluorescent protein (GFP) from the jellyfish Aequorea victoria, with that of the Ob-tobamovirus movement protein (MP) resulted in the expression of a fluorescent fusion protein (MP::GFP) that was fully biologically active in mediating the cell-to-cell spread of the Ob-virus. The MP::GFP fusion was used to follow in planta the subcellular trafficking of MP. GFP-tagged MP was transiently expressed and found to be associated with several subcellular compartments and structures including trans-wall structures, presumably plasmodesmata, and filament structures. The MP::GFP fusion can be used to monitor MP association with host proteins and structures, and for the isolation of interacting host components.
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pubmed:language |
eng
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pubmed:journal |
|
pubmed:citationSubset |
IM
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pubmed:chemical |
|
pubmed:status |
MEDLINE
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pubmed:issn |
0378-1119
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pubmed:author |
|
pubmed:issnType |
Print
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pubmed:volume |
173
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
75-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8707060-Animals,
pubmed-meshheading:8707060-Cloning, Molecular,
pubmed-meshheading:8707060-Green Fluorescent Proteins,
pubmed-meshheading:8707060-Kinetics,
pubmed-meshheading:8707060-Luminescent Proteins,
pubmed-meshheading:8707060-Plant Leaves,
pubmed-meshheading:8707060-Plant Viral Movement Proteins,
pubmed-meshheading:8707060-Plants, Toxic,
pubmed-meshheading:8707060-Protoplasts,
pubmed-meshheading:8707060-Recombinant Fusion Proteins,
pubmed-meshheading:8707060-Scyphozoa,
pubmed-meshheading:8707060-Tobacco,
pubmed-meshheading:8707060-Tobamovirus,
pubmed-meshheading:8707060-Viral Proteins
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pubmed:year |
1996
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pubmed:articleTitle |
Plant virus movement protein dynamics probed with a GFP-protein fusion.
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pubmed:affiliation |
Department of Cell Biology, Scripps Research Institute, La Jolla, CA 92037, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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