Linked Life Data

8699436

Source:http://linkedlifedata.com/resource/pubmed/id/8699436

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1996-9-5
pubmed:abstractText
Proteins secreted by sheep ovarian follicles at different stages of maturation (small healthy, large healthy or large atretic) and originating from ewes with different ovulation rates (homozygous carriers or noncarriers of the FecB gene) were analysed by two-dimensional electrophoresis (PAGE) followed by computerized image analysis. Follicles were incubated intact for 1 h to assess steroidogenesis, and then incubated for 24 h in the presence of [35S]methionine. Secreted proteins were then resolved by iso-electric focusing followed by migration on 10% acrylamide slab gels and fluorography. Incorporation of label into proteins was similar irrespective of genetic type or health status of the follicles (4-6%). Small follicles incorporated significantly less (P < 0.05) label. Over 100 spots were detected on the fluorographs. The presence of the FecB gene induced qualitative and quantitative changes in the follicular protein patterns. Frequency of detection of spots 116 and 129 was significantly higher (P < 0.01) in Fec+Fec+ compared with FecBFecB follicles (80% versus 8%). In addition, amounts of proteins present in spots 2 and 12 were increased in FecBFecB follicles, while those in spots 9 and 21 were decreased. Size and atresia affected protein patterns only quantitatively. Increased amounts of proteins in spots 1, 10, 38, 44 and 113 were associated with atresia (P < 0.05). Follicle enlargement was associated with increased (P < 0.05) amounts of proteins in spots 5 and 6 and decreased amounts of proteins in spot 16. Amounts of three proteins (33, 40 and 58) were related positively to oestradiol production in vitro before labelling.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0022-4251
pubmed:author
pubmed:issnType
Print
pubmed:volume
107
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
69-77
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Analysis by two-dimensional electrophoresis of proteins secreted by sheep ovarian follicles: effects of the FecB gene, follicle size and atresia.
pubmed:affiliation
INRA, Physiologie de la Reproduction des Mammifères Domestiques, Nouzilly, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't