rdf:type |
|
lifeskim:mentions |
|
pubmed:issue |
7
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pubmed:dateCreated |
1996-9-4
|
pubmed:abstractText |
An enterotoxigenic Escherichia coli (ETEC) strain producing a previously undescribed putative colonization factor was isolated from a child with diarrhea in India. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of bacterial heat extracts revealed a polypeptide band of 20.8 kDa when the bacteria were grown at 37 degrees C which was absent after growth at 22 degrees C. A specific rabbit antiserum raised against the purified 20.8-kDa protein bound specifically to the fimbriae, as shown by immunoelectron microscopy, and inhibited bacterial adhesion to tissue-cultured Caco-2 cells. Transformation with a recombinant plasmid harboring the cfaD gene, which encodes a positive regulator for several ETEC fimbriae, induced hyperexpression of the 20.8-kDa fimbrial subunit and a substantial increase in the proportion of bacterial cells that were fimbriated. The N-terminal amino acid sequence of the polypeptide showed 65 and 60% identity to the PCFO20 and 987P fimbriae of human and porcine ETEC, respectively. We propose the term CS20 for this new putative colonization factor of human ETEC.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
|
pubmed:citationSubset |
IM
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pubmed:chemical |
|
pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0019-9567
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pubmed:author |
|
pubmed:issnType |
Print
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pubmed:volume |
64
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2635-42
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8698489-Adhesins, Bacterial,
pubmed-meshheading:8698489-Amino Acid Sequence,
pubmed-meshheading:8698489-Animals,
pubmed-meshheading:8698489-Antibodies, Bacterial,
pubmed-meshheading:8698489-Bacterial Proteins,
pubmed-meshheading:8698489-Enterotoxins,
pubmed-meshheading:8698489-Escherichia coli,
pubmed-meshheading:8698489-Genes, Bacterial,
pubmed-meshheading:8698489-Genes, Regulator,
pubmed-meshheading:8698489-Humans,
pubmed-meshheading:8698489-Microscopy, Electron,
pubmed-meshheading:8698489-Molecular Sequence Data,
pubmed-meshheading:8698489-Molecular Weight,
pubmed-meshheading:8698489-Plasmids,
pubmed-meshheading:8698489-Rabbits,
pubmed-meshheading:8698489-Sequence Homology, Amino Acid,
pubmed-meshheading:8698489-Swine,
pubmed-meshheading:8698489-Transformation, Genetic
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pubmed:year |
1996
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pubmed:articleTitle |
Identification and characterization of CS20, a new putative colonization factor of enterotoxigenic Escherichia coli.
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pubmed:affiliation |
Centre for Internation Health, University of Bergen, Norway. Havard. Valvatne@bio.uib.no
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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