Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
1996-9-4
pubmed:abstractText
An enterotoxigenic Escherichia coli (ETEC) strain producing a previously undescribed putative colonization factor was isolated from a child with diarrhea in India. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of bacterial heat extracts revealed a polypeptide band of 20.8 kDa when the bacteria were grown at 37 degrees C which was absent after growth at 22 degrees C. A specific rabbit antiserum raised against the purified 20.8-kDa protein bound specifically to the fimbriae, as shown by immunoelectron microscopy, and inhibited bacterial adhesion to tissue-cultured Caco-2 cells. Transformation with a recombinant plasmid harboring the cfaD gene, which encodes a positive regulator for several ETEC fimbriae, induced hyperexpression of the 20.8-kDa fimbrial subunit and a substantial increase in the proportion of bacterial cells that were fimbriated. The N-terminal amino acid sequence of the polypeptide showed 65 and 60% identity to the PCFO20 and 987P fimbriae of human and porcine ETEC, respectively. We propose the term CS20 for this new putative colonization factor of human ETEC.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8698489-1100526, http://linkedlifedata.com/resource/pubmed/commentcorrection/8698489-1297915, http://linkedlifedata.com/resource/pubmed/commentcorrection/8698489-1354200, http://linkedlifedata.com/resource/pubmed/commentcorrection/8698489-1684712, http://linkedlifedata.com/resource/pubmed/commentcorrection/8698489-1774313, http://linkedlifedata.com/resource/pubmed/commentcorrection/8698489-1828771, http://linkedlifedata.com/resource/pubmed/commentcorrection/8698489-1955874, http://linkedlifedata.com/resource/pubmed/commentcorrection/8698489-1967623, http://linkedlifedata.com/resource/pubmed/commentcorrection/8698489-1968436, http://linkedlifedata.com/resource/pubmed/commentcorrection/8698489-1969831, http://linkedlifedata.com/resource/pubmed/commentcorrection/8698489-1971248, http://linkedlifedata.com/resource/pubmed/commentcorrection/8698489-1971911, http://linkedlifedata.com/resource/pubmed/commentcorrection/8698489-1981895, http://linkedlifedata.com/resource/pubmed/commentcorrection/8698489-2004811, http://linkedlifedata.com/resource/pubmed/commentcorrection/8698489-2077360, http://linkedlifedata.com/resource/pubmed/commentcorrection/8698489-2157338, http://linkedlifedata.com/resource/pubmed/commentcorrection/8698489-2180823, http://linkedlifedata.com/resource/pubmed/commentcorrection/8698489-2216800, http://linkedlifedata.com/resource/pubmed/commentcorrection/8698489-2229350, http://linkedlifedata.com/resource/pubmed/commentcorrection/8698489-2405013, http://linkedlifedata.com/resource/pubmed/commentcorrection/8698489-2456269, http://linkedlifedata.com/resource/pubmed/commentcorrection/8698489-2569152, http://linkedlifedata.com/resource/pubmed/commentcorrection/8698489-2576034, http://linkedlifedata.com/resource/pubmed/commentcorrection/8698489-2675484, http://linkedlifedata.com/resource/pubmed/commentcorrection/8698489-2864313, http://linkedlifedata.com/resource/pubmed/commentcorrection/8698489-2865086, http://linkedlifedata.com/resource/pubmed/commentcorrection/8698489-2878888, http://linkedlifedata.com/resource/pubmed/commentcorrection/8698489-2883122, http://linkedlifedata.com/resource/pubmed/commentcorrection/8698489-323375, http://linkedlifedata.com/resource/pubmed/commentcorrection/8698489-3276628, http://linkedlifedata.com/resource/pubmed/commentcorrection/8698489-336541, http://linkedlifedata.com/resource/pubmed/commentcorrection/8698489-3516874, http://linkedlifedata.com/resource/pubmed/commentcorrection/8698489-3546273, http://linkedlifedata.com/resource/pubmed/commentcorrection/8698489-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/8698489-6199307, http://linkedlifedata.com/resource/pubmed/commentcorrection/8698489-7042570, http://linkedlifedata.com/resource/pubmed/commentcorrection/8698489-776834, http://linkedlifedata.com/resource/pubmed/commentcorrection/8698489-7901165, http://linkedlifedata.com/resource/pubmed/commentcorrection/8698489-7914665, http://linkedlifedata.com/resource/pubmed/commentcorrection/8698489-8051259, http://linkedlifedata.com/resource/pubmed/commentcorrection/8698489-8094931, http://linkedlifedata.com/resource/pubmed/commentcorrection/8698489-8094932, http://linkedlifedata.com/resource/pubmed/commentcorrection/8698489-8438608, http://linkedlifedata.com/resource/pubmed/commentcorrection/8698489-942051
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0019-9567
pubmed:author
pubmed:issnType
Print
pubmed:volume
64
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2635-42
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed-meshheading:8698489-Adhesins, Bacterial, pubmed-meshheading:8698489-Amino Acid Sequence, pubmed-meshheading:8698489-Animals, pubmed-meshheading:8698489-Antibodies, Bacterial, pubmed-meshheading:8698489-Bacterial Proteins, pubmed-meshheading:8698489-Enterotoxins, pubmed-meshheading:8698489-Escherichia coli, pubmed-meshheading:8698489-Genes, Bacterial, pubmed-meshheading:8698489-Genes, Regulator, pubmed-meshheading:8698489-Humans, pubmed-meshheading:8698489-Microscopy, Electron, pubmed-meshheading:8698489-Molecular Sequence Data, pubmed-meshheading:8698489-Molecular Weight, pubmed-meshheading:8698489-Plasmids, pubmed-meshheading:8698489-Rabbits, pubmed-meshheading:8698489-Sequence Homology, Amino Acid, pubmed-meshheading:8698489-Swine, pubmed-meshheading:8698489-Transformation, Genetic
pubmed:year
1996
pubmed:articleTitle
Identification and characterization of CS20, a new putative colonization factor of enterotoxigenic Escherichia coli.
pubmed:affiliation
Centre for Internation Health, University of Bergen, Norway. Havard. Valvatne@bio.uib.no
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't