Linked Life Data

8695810

Source:http://linkedlifedata.com/resource/pubmed/id/8695810

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1996-9-5
pubmed:abstractText
Aneuploidy and lg light chain restriction were used as separate, independent tumor specific markers to study 26 patients with multiple myeloma to determine whether bone marrow B cells, as defined by CD19 expression, are clonally related to myeloma plasma cells. Specimens were characterized using multidimensional flow cytometry to identify the presence of clonality in both the B lymphoid and plasma cell populations using both surface and cytoplasmic staining with antibodies specific for kappa or lambda lg light chain In none of the patients with multiple myeloma were CD19+ cells found to be clonally restricted to kappa or lambda. The monoclonal plasma cells (MPC) were found to be uniformly negative for CD10, CD19, and CD34, while the CD19+ B lymphoid cells present within the samples expressed normal intensities and relationships of these antigens, which allowed them to serve as internal positive controls. Combined analysis of call surface antigen expression and DNA content allowed plasma cell populations to be characterized for aneuploidy without interference from normal bone marrow cells. The MPC, detected on the basis of bright CD38 expression (CD38+2), demonstrated DNA aneuploidy in 65% of cases (DNA index range of 0.9 to 1.3). These aneuploid DNA distributions had typical cell cycle profiles (including G1,S and G2+M) expected of a proliferating population. In all cases, DNA aneuploidy was confined almost entirely to the CD38+2, CD19- malignant plasma cells, while cells expressing CD19 were diploid. These results support the concept that myeloma is a disease process mediated by self-replicating, late compartments of B-cell ontogeny.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0006-4971
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
88
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
622-32
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:8695810-ADP-ribosyl Cyclase, pubmed-meshheading:8695810-Adult, pubmed-meshheading:8695810-Aged, pubmed-meshheading:8695810-Aged, 80 and over, pubmed-meshheading:8695810-Aneuploidy, pubmed-meshheading:8695810-Antigens, CD, pubmed-meshheading:8695810-Antigens, CD19, pubmed-meshheading:8695810-Antigens, CD38, pubmed-meshheading:8695810-Antigens, Differentiation, pubmed-meshheading:8695810-Antigens, Neoplasm, pubmed-meshheading:8695810-B-Lymphocyte Subsets, pubmed-meshheading:8695810-Bone Marrow, pubmed-meshheading:8695810-Cell Cycle, pubmed-meshheading:8695810-Cell Lineage, pubmed-meshheading:8695810-DNA, Neoplasm, pubmed-meshheading:8695810-Diploidy, pubmed-meshheading:8695810-Female, pubmed-meshheading:8695810-Flow Cytometry, pubmed-meshheading:8695810-Gene Rearrangement, B-Lymphocyte, Light Chain, pubmed-meshheading:8695810-Humans, pubmed-meshheading:8695810-Immunophenotyping, pubmed-meshheading:8695810-Male, pubmed-meshheading:8695810-Membrane Glycoproteins, pubmed-meshheading:8695810-Middle Aged, pubmed-meshheading:8695810-Multiple Myeloma, pubmed-meshheading:8695810-Myeloma Proteins, pubmed-meshheading:8695810-N-Glycosyl Hydrolases, pubmed-meshheading:8695810-Neoplastic Stem Cells, pubmed-meshheading:8695810-Plasma Cells
pubmed:year
1996
pubmed:articleTitle
Tumor-specific aneuploidy not detected in CD19+ B-lymphoid cells from myeloma patients in a multidimensional flow cytometric analysis.
pubmed:affiliation
Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98104, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't