Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1996-8-29
pubmed:abstractText
The pathways that regulate the S-phase events associated with the control of DNA replication are poorly understood. The bone marrow megakaryocytes are unique in that they leave the diploid (2C) state to differentiate, synthesizing 4 to 64 times the normal DNA content within a single nucleus, a process known as endomitosis. Human erythroleukemia (HEL) cells model this process, becoming polyploid during phorbol diester-induced megakaryocyte differentiation. The mitotic arrest occurring in these polyploid cells involves novel alterations in the cdk1/cyclin B1 complex: a marked reduction in cdk1 protein levels, and an elevated and sustained expression of cyclin B1. Endomitotic cells thus lack cdk1/cyclin B1-associated H1-histone kinase activity. Constitutive over-expression of cdk1 in endomitotic cells failed to re-initiate normal mitotic events even though cdk1 was present in a 10-fold excess. This was due to an inability of cyclin-B1 to physically associate with cdk1. Nonetheless, endomitotic cyclin B1 possesses immunoprecipitable H1-histone kinase activity, and specifically translocates to the nucleus. We conclude that mitosis is abrogated during endomitosis due to the absence of cdk1 and the failure to form M-phase promoting factor, resulting in a disassociation of mitosis from the completion of S-phase. Further studies on cyclin and its interacting proteins should be informative in understanding endomitosis and cell cycle control.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8688553-1191566, http://linkedlifedata.com/resource/pubmed/commentcorrection/8688553-1312467, http://linkedlifedata.com/resource/pubmed/commentcorrection/8688553-1329201, http://linkedlifedata.com/resource/pubmed/commentcorrection/8688553-1388288, http://linkedlifedata.com/resource/pubmed/commentcorrection/8688553-13962748, http://linkedlifedata.com/resource/pubmed/commentcorrection/8688553-14314398, http://linkedlifedata.com/resource/pubmed/commentcorrection/8688553-1532584, http://linkedlifedata.com/resource/pubmed/commentcorrection/8688553-1639063, http://linkedlifedata.com/resource/pubmed/commentcorrection/8688553-1652371, http://linkedlifedata.com/resource/pubmed/commentcorrection/8688553-1717476, http://linkedlifedata.com/resource/pubmed/commentcorrection/8688553-17732113, http://linkedlifedata.com/resource/pubmed/commentcorrection/8688553-1827756, http://linkedlifedata.com/resource/pubmed/commentcorrection/8688553-1827757, http://linkedlifedata.com/resource/pubmed/commentcorrection/8688553-1833062, http://linkedlifedata.com/resource/pubmed/commentcorrection/8688553-1833066, http://linkedlifedata.com/resource/pubmed/commentcorrection/8688553-1836759, http://linkedlifedata.com/resource/pubmed/commentcorrection/8688553-1846030, http://linkedlifedata.com/resource/pubmed/commentcorrection/8688553-1992340, http://linkedlifedata.com/resource/pubmed/commentcorrection/8688553-2139805, http://linkedlifedata.com/resource/pubmed/commentcorrection/8688553-2143810, http://linkedlifedata.com/resource/pubmed/commentcorrection/8688553-2205660, http://linkedlifedata.com/resource/pubmed/commentcorrection/8688553-2208288, http://linkedlifedata.com/resource/pubmed/commentcorrection/8688553-2318965, http://linkedlifedata.com/resource/pubmed/commentcorrection/8688553-2538242, http://linkedlifedata.com/resource/pubmed/commentcorrection/8688553-2541912, http://linkedlifedata.com/resource/pubmed/commentcorrection/8688553-2566918, http://linkedlifedata.com/resource/pubmed/commentcorrection/8688553-2570636, http://linkedlifedata.com/resource/pubmed/commentcorrection/8688553-2844417, http://linkedlifedata.com/resource/pubmed/commentcorrection/8688553-3033513, http://linkedlifedata.com/resource/pubmed/commentcorrection/8688553-3289755, http://linkedlifedata.com/resource/pubmed/commentcorrection/8688553-3790726, http://linkedlifedata.com/resource/pubmed/commentcorrection/8688553-4125240, http://linkedlifedata.com/resource/pubmed/commentcorrection/8688553-4208784, http://linkedlifedata.com/resource/pubmed/commentcorrection/8688553-4402332, http://linkedlifedata.com/resource/pubmed/commentcorrection/8688553-5427247, http://linkedlifedata.com/resource/pubmed/commentcorrection/8688553-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/8688553-55095, http://linkedlifedata.com/resource/pubmed/commentcorrection/8688553-6170367, http://linkedlifedata.com/resource/pubmed/commentcorrection/8688553-6187660, http://linkedlifedata.com/resource/pubmed/commentcorrection/8688553-6331437, http://linkedlifedata.com/resource/pubmed/commentcorrection/8688553-6788894, http://linkedlifedata.com/resource/pubmed/commentcorrection/8688553-6982272, http://linkedlifedata.com/resource/pubmed/commentcorrection/8688553-7059669, http://linkedlifedata.com/resource/pubmed/commentcorrection/8688553-7127400, http://linkedlifedata.com/resource/pubmed/commentcorrection/8688553-719745, http://linkedlifedata.com/resource/pubmed/commentcorrection/8688553-7737117, http://linkedlifedata.com/resource/pubmed/commentcorrection/8688553-7753858, http://linkedlifedata.com/resource/pubmed/commentcorrection/8688553-781731, http://linkedlifedata.com/resource/pubmed/commentcorrection/8688553-8070405, http://linkedlifedata.com/resource/pubmed/commentcorrection/8688553-8087848, http://linkedlifedata.com/resource/pubmed/commentcorrection/8688553-8139570, http://linkedlifedata.com/resource/pubmed/commentcorrection/8688553-8282760, http://linkedlifedata.com/resource/pubmed/commentcorrection/8688553-8423786
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1059-1524
pubmed:author
pubmed:issnType
Print
pubmed:volume
7
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
209-23
pubmed:dateRevised
2010-9-10
pubmed:meshHeading
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