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rdf:type |
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lifeskim:mentions |
umls-concept:C0021469,
umls-concept:C0027950,
umls-concept:C0038891,
umls-concept:C0040715,
umls-concept:C0086418,
umls-concept:C0213574,
umls-concept:C0441712,
umls-concept:C0596901,
umls-concept:C0599718,
umls-concept:C0599813,
umls-concept:C0599893,
umls-concept:C0599946,
umls-concept:C0897757,
umls-concept:C1135183,
umls-concept:C1280500,
umls-concept:C1417608,
umls-concept:C1417609,
umls-concept:C1522702
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pubmed:issue |
6
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pubmed:dateCreated |
1996-8-13
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pubmed:abstractText |
Surfactant has been shown to inhibit the production of reactive oxygen intermediates by various cells including alveolar macrophages and peripheral blood neutrophils. Superoxide O2-. production by the respiratory burst oxidase in isolated plasma membranes prepared from PMA-treated human neutrophils was significantly attenuated by prior treatment with native porcine surfactant. The effect was concentration dependent with half-maximal inhibition seen at approximately 0.050 mg surfactant phospholipid/ml. Kinetic analyses of the membrane-bound enzyme prepared from neutrophils stimulated by PMA in the presence or absence of surfactant demonstrated that surfactant treatment led to a decrease in the maximal velocity of O2-. production when NADPH was used as substrate, but there was no effect on enzyme substrate affinity. Immunoblotting studies demonstrated that surfactant treatment induced a decrease in the association of two oxidase components, p47phox and p67phox, with the isolated plasma membrane. In contrast, surfactant treatment of the cells did not alter the phosphorylation of p47phox. A mixture of phospholipids (phosphatidylcholine and phosphatidylglycerol in a 7:3 ratio) showed similar inhibition of the PMA-induced O2-. generation. Taken together, these data suggest the mechanism of surfactant-induced inhibition of O2-. production by human neutrophils involves attenuation of translocation of cytosolic components of the respiratory burst oxidase to the plasma membrane. The phospholipid components of surfactant appear to play a significant role in this mechanism.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8675631-1312344,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8675631-1320378,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8675631-1334078,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8675631-1564401,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8675631-1639094,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8675631-1694061,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8675631-1752956,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8675631-1848559,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8675631-1848844,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8675631-1985107,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8675631-1986776,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8675631-1993212,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8675631-2155923,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8675631-2168417,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8675631-2170384,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8675631-2171995,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8675631-2172715,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8675631-2306370,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8675631-2547247,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8675631-2619038,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8675631-2745440,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8675631-2817603,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8675631-2820982,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8675631-3000367,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8675631-3004270,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8675631-3042738,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8675631-3360806,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8675631-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8675631-576571,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8675631-577293,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8675631-6270561,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8675631-6328187,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8675631-6351681,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8675631-6425297,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8675631-6896715,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8675631-7929117,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8675631-7933425,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8675631-8031347,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8675631-8120032,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8675631-8141310,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8675631-8195693,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8675631-8200999,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8675631-8217193,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8675631-8219237,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8675631-8407934,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8675631-8420422
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Liposomes,
http://linkedlifedata.com/resource/pubmed/chemical/NADH, NADPH Oxidoreductases,
http://linkedlifedata.com/resource/pubmed/chemical/NADP,
http://linkedlifedata.com/resource/pubmed/chemical/NADPH Oxidase,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proteolipids,
http://linkedlifedata.com/resource/pubmed/chemical/Pulmonary Surfactant-Associated...,
http://linkedlifedata.com/resource/pubmed/chemical/Pulmonary Surfactants,
http://linkedlifedata.com/resource/pubmed/chemical/Superoxides,
http://linkedlifedata.com/resource/pubmed/chemical/Tetradecanoylphorbol Acetate,
http://linkedlifedata.com/resource/pubmed/chemical/neutrophil cytosol factor 67K,
http://linkedlifedata.com/resource/pubmed/chemical/neutrophil cytosolic factor 1,
http://linkedlifedata.com/resource/pubmed/chemical/superoxide-forming enzyme
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0021-9738
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
96
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2654-60
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
|
