Linked Life Data

8671331

Source:http://linkedlifedata.com/resource/pubmed/id/8671331

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1996-11-21
pubmed:abstractText
The purpose of our study was to compare the protein patterns originating from fluids of mature and immature human follicles in order to gain further insight into their biochemical composition. A total of 10 patients were stimulated for in-vitro fertilization (IVF) using different stimulation protocols. Follicular fluids were aspirated transvaginally and analysed microscopically for the presence of oocytes. Follicular fluids were stored at -18 degrees C. Samples of 500 microliters were processed for two-dimensional gel electrophoresis. Up to 60 proteins in various groups could be detected. Seven protein spots were selected for chemical analysis by cutting them out of the gels and subjecting them to internal amino acid sequencing procedures. Our results can be summarized as follows: (i) major differences were not detected between the protein patterns from the various mature follicles of a particular patient, nor were significant differences observed in the proteins derived from follicular fluids collected from the seven patients with mature follicles; (ii) considerable differences were observed in the protein patterns derived from fluids of immature compared with mature follicles. Fluid from the three patients with immature follicles contained many fewer proteins, some of which were expressed at low levels. We conclude that the observed variations in protein composition of follicles of different developmental age reflect their physiological condition and serve as biomedical markers for follicular maturity.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0268-1161
pubmed:author
pubmed:issnType
Print
pubmed:volume
11
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
798-807
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Different protein patterns derived from follicular fluid of mature and immature human follicles.
pubmed:affiliation
Department of Obstetrics and Gynecology, General Hospital, Salzburg, Austria.
pubmed:publicationType
Journal Article