Linked Life Data

8663358

Source:http://linkedlifedata.com/resource/pubmed/id/8663358

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
28
pubmed:dateCreated
1996-8-29
pubmed:databankReference
pubmed:abstractText
A yeast mutant (LT06) was isolated that showed no growth on iron-limited medium but normal growth on iron-replete medium. A gene cloned from a genomic yeast library complemented the defect, allowing growth on low iron medium. Allelic segregation analysis demonstrated that the cloned gene was the normal allele rather than a high copy suppressor. A disruption mutant was nonviable, indicating that the gene was essential. Sequence analysis and functional assays indicated that the cloned gene was identical to ERG25, a gene that codes for methyl sterol oxidase. Incubation of LT06 in low iron medium resulted in marked changes in lipid metabolism, including the accumulation of fatty acids, triglycerides, methyl sterols, and other sterol precursors. A human homologue of ERG25 was cloned, sequenced, and mapped to human chromosome 4q32-34. Analysis of the data base with both ERG25 and the human homologue resulted in the identification of a putative set of metal binding motifs with similarity to that seen in a family of membrane desaturases-hydroxylases. Western analysis using antibodies to an Erg25-GST fusion protein detected two proteins of 34 and 75 kDa. Both proteins are membrane bound and contain one N-glycosyl unit. Immunofluorescence data suggest that the proteins are present in the endoplasmic reticulum and plasma membrane. Although ERG25 transcripts are not iron regulated, there is a large increase in the concentration of transcript in the mutant LT06 grown in low iron medium. These results suggest that the enzyme is regulated not by iron but by an end product of the ergosterol pathway.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
12
pubmed:volume
271
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
16927-33
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Characterization of yeast methyl sterol oxidase (ERG25) and identification of a human homologue.
pubmed:affiliation
Division of Cell Biology and Immunology, Department of Pathology, University of Utah School of Medicine, Salt Lake City, Utah 84132, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.