Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
25
pubmed:dateCreated
1996-8-13
pubmed:abstractText
Entry into mitosis requires the coordinated action of multiple mitotic protein kinases. In this report, we investigate the involvement of protein kinase C in the control of mitosis in human cells. Treatment of synchronized HL60 cells with the highly selective protein kinase C (PKC) inhibitor chelerythrine chloride leads to profound cell cycle arrest in G2 phase. The cellular effects of chelerythrine are not due to either direct or indirect inhibition of the known mitotic regulator p34(cdc2)/cyclin B kinase. Rather, several lines of evidence demonstrate that chelerythrine-mediated G2 phase arrest results from selective inhibition and degradation of betaII protein kinase C. First, chelerythrine causes dose-dependent inhibition of betaII PKC in vitro with an IC50 identical to that for G2 phase blockade in whole cells. Second, chelerythrine specifically inhibits betaII PKC-mediated lamin B phosphorylation and mitotic nuclear lamina disassembly. Third, chelerythrine leads to selective loss of betaII PKC during G2 phase in synchronized cells. Fourth, chelerythrine mediates activation-dependent degradation of PKC, indicating that betaII PKC is selectively activated during G2 phase of cell cycle. Taken together, these data demonstrate that betaII PKC activation at G2 phase is required for mitotic nuclear lamina disassembly and entry into mitosis and that betaII PKC-mediated phosphorylation of nuclear lamin B is important in these events.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Alkaloids, http://linkedlifedata.com/resource/pubmed/chemical/Benzophenanthridines, http://linkedlifedata.com/resource/pubmed/chemical/CDC2 Protein Kinase, http://linkedlifedata.com/resource/pubmed/chemical/CDC28 Protein Kinase, S cerevisiae, http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors, http://linkedlifedata.com/resource/pubmed/chemical/Isoenzymes, http://linkedlifedata.com/resource/pubmed/chemical/Lamin Type B, http://linkedlifedata.com/resource/pubmed/chemical/Lamins, http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Phenanthridines, http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C, http://linkedlifedata.com/resource/pubmed/chemical/chelerythrine
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
21
pubmed:volume
271
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
15045-53
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:8663071-Alkaloids, pubmed-meshheading:8663071-Benzophenanthridines, pubmed-meshheading:8663071-CDC2 Protein Kinase, pubmed-meshheading:8663071-CDC28 Protein Kinase, S cerevisiae, pubmed-meshheading:8663071-Cell Cycle, pubmed-meshheading:8663071-Chromatography, Affinity, pubmed-meshheading:8663071-Dose-Response Relationship, Drug, pubmed-meshheading:8663071-Enzyme Activation, pubmed-meshheading:8663071-Enzyme Inhibitors, pubmed-meshheading:8663071-G2 Phase, pubmed-meshheading:8663071-HL-60 Cells, pubmed-meshheading:8663071-Humans, pubmed-meshheading:8663071-Isoenzymes, pubmed-meshheading:8663071-Kinetics, pubmed-meshheading:8663071-Lamin Type B, pubmed-meshheading:8663071-Lamins, pubmed-meshheading:8663071-Mitosis, pubmed-meshheading:8663071-Nuclear Proteins, pubmed-meshheading:8663071-Phenanthridines, pubmed-meshheading:8663071-Protein Kinase C
pubmed:year
1996
pubmed:articleTitle
betaII protein kinase C is required for the G2/M phase transition of cell cycle.
pubmed:affiliation
Sealy Center for Oncology and Hematology, University of Texas Medical Branch, Galveston, Texas 77555, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.