Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1996-8-2
pubmed:databankReference
pubmed:abstractText
A 2.8-kb cDNA encoding a new transcription factor (AP-2.2) has been cloned from mouse P19 embryonal carcinoma cells, in which the corresponding mRNA begins to accumulate 30 min after retinoic acid (RA) addition. The predicted protein is 449 amino acids long and exhibits approximately 65% overall identity with other AP-2-related proteins (human AP-2, mouse AP-2alpha and beta). A 96-amino-acid-long sequence, which is almost fully conserved between all these proteins, corresponds to the previously characterized human AP-2 DNA binding domain. Expression of AP-2.2 in Escherichia coli generated a protein that formed a specific complex with the AP-2 recognition site GCCN3GGC. AP-2.2 activated transcription from a reporter gene containing an AP-2 DNA binding site and acted synergistically with RARalpha to activate transcription from the CRABPII gene promoter. Transcriptional activation required the AP-2.2 amino-terminal region that contains a domain rich in proline and glutamine residues. The pattern of AP-2.2 expression in adult tissues, which is distinct from that of AP-2alpha, is essentially restricted to male and female gonads, to most if not all the squamous epithelia, and to several exocrine glands.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0014-4827
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
225
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
338-47
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:8660922-Age Factors, pubmed-meshheading:8660922-Amino Acid Sequence, pubmed-meshheading:8660922-Animals, pubmed-meshheading:8660922-Base Sequence, pubmed-meshheading:8660922-Cell Differentiation, pubmed-meshheading:8660922-Cloning, Molecular, pubmed-meshheading:8660922-DNA, Complementary, pubmed-meshheading:8660922-DNA-Binding Proteins, pubmed-meshheading:8660922-Embryonal Carcinoma Stem Cells, pubmed-meshheading:8660922-Gene Expression Regulation, pubmed-meshheading:8660922-Immunoblotting, pubmed-meshheading:8660922-In Situ Hybridization, pubmed-meshheading:8660922-Mice, pubmed-meshheading:8660922-Molecular Sequence Data, pubmed-meshheading:8660922-Neoplastic Stem Cells, pubmed-meshheading:8660922-Promoter Regions, Genetic, pubmed-meshheading:8660922-Receptors, Retinoic Acid, pubmed-meshheading:8660922-Trans-Activators, pubmed-meshheading:8660922-Transcription, Genetic, pubmed-meshheading:8660922-Transcription Factor AP-2, pubmed-meshheading:8660922-Transcription Factors, pubmed-meshheading:8660922-Tretinoin
pubmed:year
1996
pubmed:articleTitle
AP-2.2: a novel AP-2-related transcription factor induced by retinoic acid during differentiation of P19 embryonal carcinoma cells.
pubmed:affiliation
Institut de Génétique et de Biologie Moléculaire et Cellulaire, (IGBMC), CNRS/INSERM/ULP/Collège de France, Strasbourg, France. igbmc@igbmc.u-strasbg.fr
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't