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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1996-9-4
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pubmed:abstractText |
A strategy for the design of simple colorimetric assays to follow any process that involves the net generation or consumption of hydrogen ions in aqueous solutions over the pH range of 3-10 is described. This procedure relies upon the measurement of the change in absorption when a weakly or moderately buffered solution of a pH indicator is subjected to a small change in pH. Buffers and indicators are chosen with closely matching pKa values. The versatility of this type of assay technique is illustrated using three examples.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0003-2697
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
238
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
46-9
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pubmed:dateRevised |
2003-11-14
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pubmed:meshHeading |
pubmed-meshheading:8660584-Alkylating Agents,
pubmed-meshheading:8660584-Animals,
pubmed-meshheading:8660584-Buffers,
pubmed-meshheading:8660584-Colorimetry,
pubmed-meshheading:8660584-Glutathione,
pubmed-meshheading:8660584-Hydrocarbons, Halogenated,
pubmed-meshheading:8660584-Protons,
pubmed-meshheading:8660584-Solutions,
pubmed-meshheading:8660584-Spectrophotometry,
pubmed-meshheading:8660584-Trypanosoma brucei brucei,
pubmed-meshheading:8660584-Water
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pubmed:year |
1996
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pubmed:articleTitle |
Spectrophotometric assay for processes involving changes in hydrogen ion concentration in aqueous solution.
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pubmed:affiliation |
Department of Pharmacology and Developmental Therapeutics Program, Yale University School of Medicine, New Haven, Connecticut, 06520, USA.
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pubmed:publicationType |
Journal Article
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