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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1996-7-26
|
| pubmed:databankReference | |
| pubmed:abstractText |
In the bovine herpes virus-1 (BHV-1) genome, a gene equivalent to the glycoprotein k (gK)-encoding gene of other herpesviruses was identified and sequenced. The primary translation product is predicted to comprise 338 amino acids (aa) and to exhibit a molecular mass of 37.5 kDa. It possesses characteristics typical for membrane glycoproteins including a potential cleavable signal sequence, three transmembrane domains and two potential N-linked glycosylation sites. Comparison to the gK proteins of the other herpesviruses revealed aa sequence homologies of 46, 44, 53, 43, and 46% with the gK counterparts of herpes simplex viruses-1 and 2 (HSV-1 and 2), equine herpesvirus-1 (EHV-1), Marek's disease virus (MDV) and varicella zoster virus (VZV), respectively. A 30-kDa primary translation product was identified following in vitro translation of in vitro transcribed mRNA. When canine microsomal membranes were added to the translation reaction, a 38-kDa glycosylated protein was detected. Treatment with endoglycosidase F or H (endo or H) removed the glycosyl groups and reduced the apparent molecular mass of the 38-kDa glycoprotein.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0378-1119
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
12
|
| pubmed:volume |
168
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
189-93
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8654942-Amino Acid Sequence,
pubmed-meshheading:8654942-Animals,
pubmed-meshheading:8654942-Base Sequence,
pubmed-meshheading:8654942-Cattle,
pubmed-meshheading:8654942-Gene Expression,
pubmed-meshheading:8654942-Genes, Viral,
pubmed-meshheading:8654942-Genome, Viral,
pubmed-meshheading:8654942-Glycosylation,
pubmed-meshheading:8654942-Herpesviridae,
pubmed-meshheading:8654942-Herpesvirus 1, Bovine,
pubmed-meshheading:8654942-Herpesvirus 1, Equid,
pubmed-meshheading:8654942-Herpesvirus 1, Human,
pubmed-meshheading:8654942-Horses,
pubmed-meshheading:8654942-Humans,
pubmed-meshheading:8654942-Microsomes,
pubmed-meshheading:8654942-Molecular Sequence Data,
pubmed-meshheading:8654942-Protein Biosynthesis,
pubmed-meshheading:8654942-RNA, Messenger,
pubmed-meshheading:8654942-Sequence Homology, Amino Acid,
pubmed-meshheading:8654942-Viral Proteins
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
Sequence and expression of a bovine herpesvirus-1 gene homologous to the glycoprotein K-encoding gene of herpes simplex virus-1.
|
| pubmed:affiliation |
Veterinary Infectious Disease Organization, University of Saskatchewan, Saskatoon, Canada.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|