Linked Life Data

8652823

Source:http://linkedlifedata.com/resource/pubmed/id/8652823

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1996-7-31
pubmed:abstractText
Human myeloid progenitor cells temporarily express HLA class II molecules during the differentiation pathway to granulocytes and macrophages. The significance of major histocompatibility complex (MHC) class II molecules at this stage of development is unknown. As a first stop of inquiry into their function, we have characterized the profile of major self-peptides bound to the HLA-DR molecules expressed by KG-1 cells, a line that shares many of the phenotypic characteristics of colony-forming unit-granulocyte-macrophage progenitors. Searches of protein data bases showed that all matching peptides bound to the HLA-DR molecules of KG-1 cells corresponded to intracellular, rather than exogenous or transmembrane, precursor proteins. Because the absence of a conventional self-peptide repertoire could be related to altered trafficking of class II molecules, the biosynthesis of HLA-DR and the invariant chain proteins was determined. The MHC class II associated invariant chain protein is synthesized normally in KG-1 cells, but processed fragments of invariant chain, class II-associated invariant chain peptides (CLIPs), occupy the antigen-binding groove of KG-1 class II molecules at a much lower frequency compared with that of mature antigen-presenting cells. Low CLIP occupancy of HLA-DR is a characteristic shared by KG-1 cells, normal CD34+ progenitor cells, and HLA-DR+ breast carcinoma cells. The unusual profile of MHC class II bound peptides and the low level of CLIP bound to HLA-DR suggest that the antigen-processing pathway of KG-1 is different from that characterized in professional antigen-presenting cells and that exogenous antigen-processing may be a developmentally acquired characteristic in the myeloid lineage.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0006-4971
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
87
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5104-12
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:8652823-Amino Acid Sequence, pubmed-meshheading:8652823-Antigen Presentation, pubmed-meshheading:8652823-Antigens, Differentiation, B-Lymphocyte, pubmed-meshheading:8652823-Antigens, Neoplasm, pubmed-meshheading:8652823-Autoantigens, pubmed-meshheading:8652823-Breast Neoplasms, pubmed-meshheading:8652823-Cell Differentiation, pubmed-meshheading:8652823-Female, pubmed-meshheading:8652823-HLA-DR Antigens, pubmed-meshheading:8652823-Hematopoietic Stem Cells, pubmed-meshheading:8652823-Histocompatibility Antigens Class II, pubmed-meshheading:8652823-Humans, pubmed-meshheading:8652823-Intracellular Fluid, pubmed-meshheading:8652823-Leukemia, Myeloid, Acute, pubmed-meshheading:8652823-Molecular Sequence Data, pubmed-meshheading:8652823-Peptide Fragments, pubmed-meshheading:8652823-Self Tolerance, pubmed-meshheading:8652823-Sequence Alignment, pubmed-meshheading:8652823-Tumor Cells, Cultured
pubmed:year
1996
pubmed:articleTitle
Predominant HLA-class II bound self-peptides of a hematopoietic progenitor cell line are derived from intracellular proteins.
pubmed:affiliation
Department of Pathology, College of Physicians and Surgeons of Columbia University, New York, NY, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't