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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1996-7-26
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pubmed:abstractText |
Surfactant protein B (SP-B) is critical to the biophysical function of surfactant. To characterize its metabolism in vivo in the newborn, we administered [35S]methionine and [3H]palmitate to newborn rabbits intravascularly. Three groups of 4 rabbits per group were killed at each of 4 time points followed by isolation of SP-B from alveolar wash and lamellar bodies. The labeling kinetics for alveolar wash associated SP-B and saturated phosphatidylcholine (Sat PC) had similar patterns. To characterize SP-B clearance from the airspace, rabbit SP-B was iodinated, mixed with [14C]dipalmitoylphosphatidylcholine and given by intratracheal injection. Alveolar washes and lamellar bodies were recovered from 4 animals at each of 7 time points. Both SP-B and Sat PC were cleared slowly from the total lung (half-life values approximately 25 h). However, SP-B was cleared more rapidly from the airspaces than was Sat PC. The ratio of [125I]SP-B to [14C]Sat PC in lamellar bodies increased 2-fold by 8 h. These results support the concept of linked secretion and clearance pathways for SP-B and Sat PC, although small differences in reuptake were detected.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1,2-Dipalmitoylphosphatidylcholine,
http://linkedlifedata.com/resource/pubmed/chemical/Methionine,
http://linkedlifedata.com/resource/pubmed/chemical/Palmitic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Palmitic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Proteolipids,
http://linkedlifedata.com/resource/pubmed/chemical/Pulmonary Surfactants
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0006-3002
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
19
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pubmed:volume |
1300
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
97-102
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:8652643-1,2-Dipalmitoylphosphatidylcholine,
pubmed-meshheading:8652643-Animals,
pubmed-meshheading:8652643-Animals, Newborn,
pubmed-meshheading:8652643-Bronchoalveolar Lavage Fluid,
pubmed-meshheading:8652643-Kinetics,
pubmed-meshheading:8652643-Lung,
pubmed-meshheading:8652643-Methionine,
pubmed-meshheading:8652643-Palmitic Acid,
pubmed-meshheading:8652643-Palmitic Acids,
pubmed-meshheading:8652643-Proteolipids,
pubmed-meshheading:8652643-Pulmonary Alveoli,
pubmed-meshheading:8652643-Pulmonary Surfactants,
pubmed-meshheading:8652643-Rabbits,
pubmed-meshheading:8652643-Trachea
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pubmed:year |
1996
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pubmed:articleTitle |
Surfactant protein B metabolism in newborn rabbits.
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pubmed:affiliation |
Department of Pediatrics, Harbor-UCLA Medical Center, Torrance, CA 90509, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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