Linked Life Data

8651450

Source:http://linkedlifedata.com/resource/pubmed/id/8651450

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1996-7-19
pubmed:abstractText
Acute ethanol, in both man and cats, decreases contractility of both lower esophageal sphincter (LES) and smooth muscle portion of the lower esophageal (LE) body. Because these inhibitory effects were not abolished, in cats, by cervical vagotomy or intravenous tetrodotoxin, we surmised a direct inhibitory effect of ethanol on muscle cells. Accordingly, to test this possibility, we exposed isolated, esophageal smooth muscle cells (LES and LE) to ethanol (0-150 mM) for 0 to 40 min, and then a contractile agent, carbachol, or its vehicle was added. Thirty seconds later, cells were fixed and cell shortening was measured as an index of contractility. In the absence of ethanol, carbachol dose-dependently induced shortening of muscle cells from both LE and LES. Ethanol significantly attenuated carbachol-induced maximal shortening of cells from both LE and LES. Potency for carbachol in LES (but not LE) was also decreased by ethanol. Isolated muscle cells remained viable after incubation with ethanol. Thus inhibition by ethanol: can occur directly on esophageal muscle; occurs at pharmacologically relevant ethanol concentrations; and is not simply caused by cytotoxicity of ethanol.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0145-6008
pubmed:author
pubmed:issnType
Print
pubmed:volume
20
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
180-4
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Ethanol at pharmacologically relevant concentrations inhibits contractility of isolated smooth muscle cells of cat esophagus.
pubmed:affiliation
Department of Medicine, Loyola University, Maywood, Illinois 60153, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.