Linked Life Data

8650213

Source:http://linkedlifedata.com/resource/pubmed/id/8650213

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1996-7-25
pubmed:databankReference
pubmed:abstractText
A new and highly effective method, termed suppression subtractive hybridization (SSH), has been developed for the generation of subtracted cDNA libraries. It is based primarily on a recently described technique called suppression PCR and combines normalization and subtraction in a single procedure. The normalization step equalizes the abundance of cDNAs within the target population and the subtraction step excludes the common sequences between the target and driver populations. In a model system, the SSH technique enriched for rare sequences over 1,000-fold in one round of subtractive hybridization. We demonstrate its usefulness by generating a testis-specific cDNA library and by using the subtracted cDNA mixture as a hybridization probe to identify homologous sequences in a human Y chromosome cosmid library. The human DNA inserts in the isolated cosmids were further confirmed to be expressed in a testis-specific manner. These results suggest that the SSH technique is applicable to many molecular genetic and positional cloning studies for the identification of disease, developmental, tissue-specific, or other differentially expressed genes.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8650213-1284595, http://linkedlifedata.com/resource/pubmed/commentcorrection/8650213-1354393, http://linkedlifedata.com/resource/pubmed/commentcorrection/8650213-1383934, http://linkedlifedata.com/resource/pubmed/commentcorrection/8650213-1722336, http://linkedlifedata.com/resource/pubmed/commentcorrection/8650213-1766870, http://linkedlifedata.com/resource/pubmed/commentcorrection/8650213-2216762, http://linkedlifedata.com/resource/pubmed/commentcorrection/8650213-2307059, http://linkedlifedata.com/resource/pubmed/commentcorrection/8650213-2339063, http://linkedlifedata.com/resource/pubmed/commentcorrection/8650213-2456582, http://linkedlifedata.com/resource/pubmed/commentcorrection/8650213-6199676, http://linkedlifedata.com/resource/pubmed/commentcorrection/8650213-6326111, http://linkedlifedata.com/resource/pubmed/commentcorrection/8650213-6688681, http://linkedlifedata.com/resource/pubmed/commentcorrection/8650213-7501479, http://linkedlifedata.com/resource/pubmed/commentcorrection/8650213-7731798, http://linkedlifedata.com/resource/pubmed/commentcorrection/8650213-7838717, http://linkedlifedata.com/resource/pubmed/commentcorrection/8650213-8438152, http://linkedlifedata.com/resource/pubmed/commentcorrection/8650213-8524670
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
11
pubmed:volume
93
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6025-30
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Suppression subtractive hybridization: a method for generating differentially regulated or tissue-specific cDNA probes and libraries.
pubmed:affiliation
CLONTECH Laboratories, Inc., Palo Alto, CA 94303, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.