Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1996-7-22
pubmed:abstractText
Telomerase is a ribonucleoprotein whose activity has been detected in germ line cells, immortal cells, and most cancer cells. Except in stem cells, which have a low level of telomerase activity, its activity is absent from normal somatic tissues. Understanding the regulation of telomerase activity is critical for the development of potential tools for the diagnosis and treatment of cancer. Using the telomeric repeat amplification protocol, we found that immortal, telomerase-positive, pseudodiploid human cells (HT1080 and HL60 cells) sorted by flow repressed in quiescent cells. This was true whether quiescence was induced by contact inhibition (NIH 3T3 mouse cells), growth factor removal (bromodeoxyuridine-blocked mouse myoblasts), reexpression of cellular senescence (the reversibly immortalized IDH4 cells), or irreversible cell differentiation (HL60 promyelocytic leukemia cells and C2C12 mouse myoblasts). Taken together, these results indicate that telomerase is active throughout the cell in dividing, immortal cells but that its activity is repressed in cells that exit the cell cycle. This suggests that quiescent stem cells that have the potential to express telomerase may remain unaffected by potential antitelomerase cancer therapies.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8649404-1333985, http://linkedlifedata.com/resource/pubmed/commentcorrection/8649404-1406665, http://linkedlifedata.com/resource/pubmed/commentcorrection/8649404-1438199, http://linkedlifedata.com/resource/pubmed/commentcorrection/8649404-1496553, http://linkedlifedata.com/resource/pubmed/commentcorrection/8649404-1537344, http://linkedlifedata.com/resource/pubmed/commentcorrection/8649404-1582420, http://linkedlifedata.com/resource/pubmed/commentcorrection/8649404-2342578, http://linkedlifedata.com/resource/pubmed/commentcorrection/8649404-2463488, http://linkedlifedata.com/resource/pubmed/commentcorrection/8649404-2655926, http://linkedlifedata.com/resource/pubmed/commentcorrection/8649404-3413114, http://linkedlifedata.com/resource/pubmed/commentcorrection/8649404-7135161, http://linkedlifedata.com/resource/pubmed/commentcorrection/8649404-7479015, http://linkedlifedata.com/resource/pubmed/commentcorrection/8649404-7545955, http://linkedlifedata.com/resource/pubmed/commentcorrection/8649404-7561072, http://linkedlifedata.com/resource/pubmed/commentcorrection/8649404-7568077, http://linkedlifedata.com/resource/pubmed/commentcorrection/8649404-7585042, http://linkedlifedata.com/resource/pubmed/commentcorrection/8649404-7605428, http://linkedlifedata.com/resource/pubmed/commentcorrection/8649404-7613096, http://linkedlifedata.com/resource/pubmed/commentcorrection/8649404-7630414, http://linkedlifedata.com/resource/pubmed/commentcorrection/8649404-7666478, http://linkedlifedata.com/resource/pubmed/commentcorrection/8649404-7718893, http://linkedlifedata.com/resource/pubmed/commentcorrection/8649404-7761406, http://linkedlifedata.com/resource/pubmed/commentcorrection/8649404-7796395, http://linkedlifedata.com/resource/pubmed/commentcorrection/8649404-7847852, http://linkedlifedata.com/resource/pubmed/commentcorrection/8649404-8032197, http://linkedlifedata.com/resource/pubmed/commentcorrection/8649404-8039513, http://linkedlifedata.com/resource/pubmed/commentcorrection/8649404-8159676, http://linkedlifedata.com/resource/pubmed/commentcorrection/8649404-8163557, http://linkedlifedata.com/resource/pubmed/commentcorrection/8649404-8175699, http://linkedlifedata.com/resource/pubmed/commentcorrection/8649404-8422682, http://linkedlifedata.com/resource/pubmed/commentcorrection/8649404-8618897, http://linkedlifedata.com/resource/pubmed/commentcorrection/8649404-8934879, http://linkedlifedata.com/resource/pubmed/commentcorrection/8649404-8991544, http://linkedlifedata.com/resource/pubmed/commentcorrection/8649404-9415184
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0270-7306
pubmed:author
pubmed:issnType
Print
pubmed:volume
16
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2932-9
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Regulation of telomerase activity in immortal cell lines.
pubmed:affiliation
Department of Cell Biology and Neuroscience, University of Texas Southwestern Medical Center, Dallas 75235-9039, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't