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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1996-7-23
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pubmed:abstractText |
The B-chain homodimer isoform of platelet-derived growth factor (PDGF) binds with high affinity both to alpha- and to beta-receptors. In order to localize amino acid residues in PDGF-BB of differential importance for the binding to the two receptors, PDGF-BB mutants were analyzed in which single amino acid residues were changed to alanine residues. We found that Phe-118 in loop 1 of the PDGF B-chain is crucial for binding to both receptors, and that the surrounding amino acids, Asn-117 and Leu-119, appear to be important primarily for binding to the beta-receptor. In contrast, Lys-161 in loop 3 was found to be more important for binding to alpha-receptors than beta-receptors. Previous studies have shown that the receptor binding epitope of PDGF-BB is composed mainly of loops 1 and 3; the findings of the present study show that the alpha- and beta-receptors interact with different amino acid residues in these regions.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0014-5793
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
6
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pubmed:volume |
385
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
181-4
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:8647246-Amino Acid Sequence,
pubmed-meshheading:8647246-Amino Acids,
pubmed-meshheading:8647246-Animals,
pubmed-meshheading:8647246-Binding, Competitive,
pubmed-meshheading:8647246-Binding Sites,
pubmed-meshheading:8647246-Cell Line,
pubmed-meshheading:8647246-Molecular Sequence Data,
pubmed-meshheading:8647246-Mutagenesis, Site-Directed,
pubmed-meshheading:8647246-Mutation,
pubmed-meshheading:8647246-Platelet-Derived Growth Factor,
pubmed-meshheading:8647246-Protein Binding,
pubmed-meshheading:8647246-Protein Conformation,
pubmed-meshheading:8647246-Receptor, Platelet-Derived Growth Factor alpha,
pubmed-meshheading:8647246-Receptor, Platelet-Derived Growth Factor beta,
pubmed-meshheading:8647246-Receptors, Platelet-Derived Growth Factor,
pubmed-meshheading:8647246-Recombinant Proteins
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pubmed:year |
1996
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pubmed:articleTitle |
Identification of three amino acid residues in the B-chain of platelet-derived growth factor with different importance for binding to PDGF alpha- and beta-receptors.
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pubmed:affiliation |
Ludwig Institute for Cancer Research, Biomedical Center, Uppsala, Sweden.
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pubmed:publicationType |
Journal Article
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