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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1996-7-18
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pubmed:abstractText |
15-lipoxygenase (15-LO) expression in artery wall cells has been demonstrated during the development of atherosclerosis in various animal models. We examined whether the expression of 15-LO in aortic endothelial cells affects the gene expression of the adhesion molecule, vascular cell adhesion molecule-1 (VCAM-1). Transient transfection of human 15-LO cDNA into bovine aortic endothelial cells led to the expression of 15-LO protein and enzymatic activity. We studied the induction of VCAM-1 mRNA in these cells. 15-LO expressing cells showed no detectable levels of VCAM-1 message. However, when TNF was added to these cells there was a synergistic increase in VCAM-1 expression relative to cells that were transfected with control plasmid pcDNA I. Our data suggest that 15-LO expression in aortic endothelium may amplify the expression of VCAM-1 induced by inflammatory stimulus during atherogenesis.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
18
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pubmed:volume |
220
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
310-4
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:8645302-Animals,
pubmed-meshheading:8645302-Antithrombins,
pubmed-meshheading:8645302-Aorta,
pubmed-meshheading:8645302-Arachidonate 15-Lipoxygenase,
pubmed-meshheading:8645302-Cattle,
pubmed-meshheading:8645302-Cells, Cultured,
pubmed-meshheading:8645302-Endothelium, Vascular,
pubmed-meshheading:8645302-Gene Expression,
pubmed-meshheading:8645302-Gene Expression Regulation,
pubmed-meshheading:8645302-Humans,
pubmed-meshheading:8645302-Linoleic Acids,
pubmed-meshheading:8645302-RNA, Messenger,
pubmed-meshheading:8645302-Transfection,
pubmed-meshheading:8645302-Tumor Necrosis Factor-alpha,
pubmed-meshheading:8645302-Vascular Cell Adhesion Molecule-1
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pubmed:year |
1996
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pubmed:articleTitle |
Transient overexpression of human 15-lipoxygenase in aortic endothelial cells enhances tumor necrosis factor-induced vascular cell adhesion molecule-1 gene expression.
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pubmed:affiliation |
Department of Atherosclerosis Therapeutics, Parke-Davis Pharmaceutical Research, Division of Warner-Lambert Company, Ann Arbor, Michigan, 48105, USA.
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pubmed:publicationType |
Journal Article
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