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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1996-7-16
|
| pubmed:abstractText |
We have studied the proliferation of cells in two models of chemical hepatocarcinogenesis. The cells were genetically labeled in vivo using retrovirally mediated transfer of the Escherichia coli beta-galactosidase marker gene coupled to a nuclear localization signal (nls-lacZ gene). In the first carcinogenic model, rats were fed a choline-deficient diet containing 2-acetylaminofluorene, and their livers were perfused with recombinant retrovirus at the onset of oval cell proliferation. The second model was based on the administration of diethylnitrosamine coupled with a partial hepatectomy and is thought to induce cancer with no involvement of oval cells. Analysis of beta-galactosidase expression in the liver at various times after gene transfer revealed the presence of large clusters of positive cells in both models. Moreover, the beta-galactosidase-positive cells displayed morphologic, antigenic, and enzymatic profiles consistent with a hepatocyte phenotype. Our results, therefore, provide evidence for a strikingly similar clonal proliferation of apparently normal hepatocytes during the course of 2-acetylaminofluorene- as well as diethylnitrosamine-induced liver carcinogenesis.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/2-Acetylaminofluorene,
http://linkedlifedata.com/resource/pubmed/chemical/Carcinogens,
http://linkedlifedata.com/resource/pubmed/chemical/Diethylnitrosamine,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Markers, Biological,
http://linkedlifedata.com/resource/pubmed/chemical/beta-Galactosidase
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0023-6837
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
74
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
871-81
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8642783-2-Acetylaminofluorene,
pubmed-meshheading:8642783-Animals,
pubmed-meshheading:8642783-Carcinogens,
pubmed-meshheading:8642783-Cell Count,
pubmed-meshheading:8642783-Cell Division,
pubmed-meshheading:8642783-Cell Line,
pubmed-meshheading:8642783-Cell Lineage,
pubmed-meshheading:8642783-Diethylnitrosamine,
pubmed-meshheading:8642783-Disease Models, Animal,
pubmed-meshheading:8642783-Gene Transfer Techniques,
pubmed-meshheading:8642783-Genetic Vectors,
pubmed-meshheading:8642783-Liver,
pubmed-meshheading:8642783-Liver Neoplasms, Experimental,
pubmed-meshheading:8642783-Male,
pubmed-meshheading:8642783-Rats,
pubmed-meshheading:8642783-Rats, Wistar,
pubmed-meshheading:8642783-Retroviridae,
pubmed-meshheading:8642783-Time Factors,
pubmed-meshheading:8642783-Tumor Markers, Biological,
pubmed-meshheading:8642783-beta-Galactosidase
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
In vivo cell lineage analysis during chemical hepatocarcinogenesis using retroviral-mediated gene transfer.
|
| pubmed:affiliation |
INSERM U370, Paris, France.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|