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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
1996-7-18
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pubmed:abstractText |
The ribozyme from Bacillus subtilis RNase P (P RNA) recognizes an RNA structure consisting of the acceptor stem and the T stem-loop of tRNA substrates. An in vitro selection experiment was carried out to obtain potential RNA substrates that may interact with the P RNA differently from the tRNA substrate. Using a P RNA-derived ribozyme that contains most, if not all, of the structural elements thought to be involved in active site formation of P RNA, but lacks the putative binding site for the T stem-loop of tRNA, a single RNA substrate was isolated after nine rounds of selection. This RNA is a competent substrate for the ribozyme used in selection as well as for the full-length P RNA. Biochemical characterization shows that this selected substrate interacts at a different site compared with the tRNA substrate. The selection experiment also identified a self-cleaving RNA seemingly different from other known ribozymes. These results indicate that a biological ribozyme can contain different binding sites for different RNA substrates. This alternate binding site model suggests a simple mechanism for evolving existing ribozymes to recognize RNA substrates of diverse structures.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8641290-1373649,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8641290-1696319,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8641290-1697102,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8641290-1716459,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8641290-1741379,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8641290-2197983,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8641290-2446263,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8641290-2456074,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8641290-2468070,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8641290-2660003,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8641290-7489486,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8641290-7511814,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8641290-7515186,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8641290-7521296,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8641290-7521297,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8641290-7524035,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8641290-7524073,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8641290-7525270,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8641290-7541243,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8641290-7597035,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8641290-7816622,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8641290-7916700,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8641290-8312258,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8641290-8618931
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0261-4189
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
15
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2249-55
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1996
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pubmed:articleTitle |
Multiple substrate binding sites in the ribozyme from Bacillus subtilis RNase P.
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pubmed:affiliation |
Department of Biochemistry and Molecular Biology, University of Chicago, IL 60637, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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