Linked Life Data

8636081

Source:http://linkedlifedata.com/resource/pubmed/id/8636081

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1996-7-9
pubmed:abstractText
The orphan nuclear receptor, steroidogenic factor-1 (SF-1), is expressed in the pituitary and in the gonadotrope precursor cell line, alphaT3-1, where it is believed to enhance expression of the common gonadotropin alpha-subunit gene through transactivation of the gonadotrope-specific element (GSE). Sequence analysis of the rat luteinizing hormone beta-subunit (LH beta) gene promoter revealed the presence of a consensus GSE at -127 to -119 (TGACCTTGT). We have demonstrated the ability of SF-1 to bind specifically to this putative GSE sequence by electrophoretic mobility shift assay, utilizing both alphaT3-1 nuclear extracts and in vitro translated SF-1. In addition, mutation of the putative LHbeta-GSE (TGAAATTGT) eliminated specific DNA binding. To examine the ability of SF-1 to enhance LHbeta promoter activity, CV-1 cells, which lack endogenous SF-1, were cotransfected with an SF-1-containing expression vector and an LHbeta-luciferase reporter construct. When cotransfected with -209/+5 of the LHbeta promoter, SF-1 increased luciferase activity by 56-fold. SF-1 responsiveness was markedly diminished with loss of the putative GSE region in deletion constructs and in the presence of a two base pair mutation, analogous to the mutation which eliminated DNA binding. Finally, the LHbeta-GSE was able to confer SF-1 responsiveness on a heterologous minimal growth hormone promoter, GH50 (57-fold). We conclude that SF-1 both binds to and transactivates the rat LHbeta promoter. These data suggest that SF-1 may participate in the expression of the LHbeta gene by the gonadotrope.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
22
pubmed:volume
271
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6645-50
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:8636081-Animals, pubmed-meshheading:8636081-Base Sequence, pubmed-meshheading:8636081-Cell Line, pubmed-meshheading:8636081-DNA, pubmed-meshheading:8636081-DNA-Binding Proteins, pubmed-meshheading:8636081-Fushi Tarazu Transcription Factors, pubmed-meshheading:8636081-Haplorhini, pubmed-meshheading:8636081-Homeodomain Proteins, pubmed-meshheading:8636081-Luteinizing Hormone, pubmed-meshheading:8636081-Mice, pubmed-meshheading:8636081-Molecular Sequence Data, pubmed-meshheading:8636081-Mutation, pubmed-meshheading:8636081-Promoter Regions, Genetic, pubmed-meshheading:8636081-Protein Biosynthesis, pubmed-meshheading:8636081-Rats, pubmed-meshheading:8636081-Receptors, Cytoplasmic and Nuclear, pubmed-meshheading:8636081-Steroidogenic Factor 1, pubmed-meshheading:8636081-Transcription Factors
pubmed:year
1996
pubmed:articleTitle
Stimulation of luteinizing hormone beta gene promoter activity by the orphan nuclear receptor, steroidogenic factor-1.
pubmed:affiliation
Division of Genetics, Department of Medicine, Brigham and Women's Hospital and Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts 02115, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't